PUBLICATION

RAD marker microarrays enable rapid mapping of zebrafish mutations

Authors
Miller, M.R., Atwood, T.S., Eames, B.F., Eberhart, J.K., Yan, Y.L., Postlethwait, J.H., and Johnson, E.A.
ID
ZDB-PUB-070625-12
Date
2007
Source
Genome biology   8(6): R105 (Journal)
Registered Authors
Eberhart, Johann, Postlethwait, John H., Yan, Yi-Lin
Keywords
none
MeSH Terms
  • Animals
  • Chromosome Mapping/economics
  • Chromosome Mapping/methods*
  • Female
  • Male
  • Mutation
  • Oligonucleotide Array Sequence Analysis/methods
  • Polymerase Chain Reaction/methods
  • Restriction Mapping
  • Zebrafish/genetics*
PubMed
17553171 Full text @ Genome Biol.
Abstract
We constructed a RAD marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and an attractive option for mapping in other organisms.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Errata and Notes