PUBLICATION
RAD marker microarrays enable rapid mapping of zebrafish mutations
- Authors
- Miller, M.R., Atwood, T.S., Eames, B.F., Eberhart, J.K., Yan, Y.L., Postlethwait, J.H., and Johnson, E.A.
- ID
- ZDB-PUB-070625-12
- Date
- 2007
- Source
- Genome biology 8(6): R105 (Journal)
- Registered Authors
- Eberhart, Johann, Postlethwait, John H., Yan, Yi-Lin
- Keywords
- none
- MeSH Terms
-
- Zebrafish/genetics*
- Oligonucleotide Array Sequence Analysis/methods
- Animals
- Male
- Polymerase Chain Reaction/methods
- Female
- Mutation
- Restriction Mapping
- Chromosome Mapping/economics
- Chromosome Mapping/methods*
- PubMed
- 17553171 Full text @ Genome Biol.
Citation
Miller, M.R., Atwood, T.S., Eames, B.F., Eberhart, J.K., Yan, Y.L., Postlethwait, J.H., and Johnson, E.A. (2007) RAD marker microarrays enable rapid mapping of zebrafish mutations. Genome biology. 8(6):R105.
Abstract
We constructed a RAD marker microarray to facilitate rapid genetic mapping of zebrafish mutations. Using these microarrays with a bulk segregant approach, we localized previously unmapped mutations to genomic regions just a few centiMorgans in length. Furthermore, we developed an approach to assay individual RAD markers in pooled populations and refined one region. The RAD approach is highly effective for genetic mapping in zebrafish and an attractive option for mapping in other organisms.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping