PUBLICATION
Caveolin-1 is required for lateral line neuromast and notochord development
- Authors
- Nixon, S.J., Carter, A., Wegner, J., Ferguson, C., Floetenmeyer, M., Riches, J., Key, B., Westerfield, M., and Parton, R.G.
- ID
- ZDB-PUB-070625-10
- Date
- 2007
- Source
- Journal of Cell Science 120(13): 2151-2161 (Journal)
- Registered Authors
- Key, Brian, Wegner, Jeremy, Westerfield, Monte
- Keywords
- Caveolae, Caveolin-1, Neuromast, Notochord, Zebrafish
- MeSH Terms
-
- Animals
- Caveolae/metabolism*
- Caveolae/ultrastructure
- Caveolin 1/biosynthesis*
- Caveolin 3/biosynthesis
- Caveolin 3/genetics
- Gene Expression Regulation, Developmental/physiology*
- Heart/embryology
- Notochord/embryology*
- Notochord/ultrastructure
- Signal Transduction/physiology
- Somites/metabolism
- Somites/ultrastructure
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- PubMed
- 17550965 Full text @ J. Cell Sci.
Citation
Nixon, S.J., Carter, A., Wegner, J., Ferguson, C., Floetenmeyer, M., Riches, J., Key, B., Westerfield, M., and Parton, R.G. (2007) Caveolin-1 is required for lateral line neuromast and notochord development. Journal of Cell Science. 120(13):2151-2161.
Abstract
Caveolae have been linked to diverse cellular functions and to many disease states. In this study we have used zebrafish to examine the role of caveolin-1 and caveolae during early embryonic development. During development, expression is apparent in a number of tissues including Kupffer's vesicle, tailbud, intersomite boundaries, heart, branchial arches, pronephric ducts and periderm. Particularly strong expression is observed in the sensory organs of the lateral line, the neuromasts and in the notochord where it overlaps with expression of caveolin-3. Morpholino-mediated downregulation of Cav1alpha caused a dramatic inhibition of neuromast formation. Detailed ultrastructural analysis, including electron tomography of the notochord, revealed that the central regions of the notochord has the highest density of caveolae of any embryonic tissue comparable to the highest density observed in any vertebrate tissue. In addition, Cav1alpha downregulation caused disruption of the notochord, an effect that was enhanced further by Cav3 knockdown. These results indicate an essential role for caveolin and caveolae in this vital structural and signalling component of the embryo.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping