PUBLICATION

Caveolin-1 is required for lateral line neuromast and notochord development

Authors
Nixon, S.J., Carter, A., Wegner, J., Ferguson, C., Floetenmeyer, M., Riches, J., Key, B., Westerfield, M., and Parton, R.G.
ID
ZDB-PUB-070625-10
Date
2007
Source
Journal of Cell Science   120(13): 2151-2161 (Journal)
Registered Authors
Key, Brian, Wegner, Jeremy, Westerfield, Monte
Keywords
Caveolae, Caveolin-1, Neuromast, Notochord, Zebrafish
MeSH Terms
  • Animals
  • Caveolae/metabolism*
  • Caveolae/ultrastructure
  • Caveolin 1/biosynthesis*
  • Caveolin 3/biosynthesis
  • Caveolin 3/genetics
  • Gene Expression Regulation, Developmental/physiology*
  • Heart/embryology
  • Notochord/embryology*
  • Notochord/ultrastructure
  • Signal Transduction/physiology
  • Somites/metabolism
  • Somites/ultrastructure
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/biosynthesis*
  • Zebrafish Proteins/genetics
PubMed
17550965 Full text @ J. Cell Sci.
Abstract
Caveolae have been linked to diverse cellular functions and to many disease states. In this study we have used zebrafish to examine the role of caveolin-1 and caveolae during early embryonic development. During development, expression is apparent in a number of tissues including Kupffer's vesicle, tailbud, intersomite boundaries, heart, branchial arches, pronephric ducts and periderm. Particularly strong expression is observed in the sensory organs of the lateral line, the neuromasts and in the notochord where it overlaps with expression of caveolin-3. Morpholino-mediated downregulation of Cav1alpha caused a dramatic inhibition of neuromast formation. Detailed ultrastructural analysis, including electron tomography of the notochord, revealed that the central regions of the notochord has the highest density of caveolae of any embryonic tissue comparable to the highest density observed in any vertebrate tissue. In addition, Cav1alpha downregulation caused disruption of the notochord, an effect that was enhanced further by Cav3 knockdown. These results indicate an essential role for caveolin and caveolae in this vital structural and signalling component of the embryo.
Genes / Markers
Figures
Show all Figures
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping