PUBLICATION

Cloning, expression, and functional characterization of zebrafish Mist1

Authors
Guo, X., Cheng, L., Liu, Y., Fan, W., and Lu, D.
ID
ZDB-PUB-070614-16
Date
2007
Source
Biochemical and Biophysical Research Communications   359(1): 20-26 (Journal)
Registered Authors
Keywords
Mist1, Basic helix–loop–helix (bHLH), Exocrine pancreas, Development, Zebrafish
MeSH Terms
  • Amino Acid Sequence
  • Animals
  • Basic Helix-Loop-Helix Transcription Factors/chemistry*
  • Basic Helix-Loop-Helix Transcription Factors/physiology*
  • Cloning, Molecular
  • Drosophila Proteins/chemistry*
  • Drosophila Proteins/physiology*
  • Embryonic Development/physiology*
  • Evolution, Molecular
  • Gene Expression Regulation, Developmental/physiology
  • Molecular Sequence Data
  • Organ Specificity
  • Pancreas/embryology*
  • Pancreas/physiology*
  • Sequence Homology, Amino Acid
  • Tissue Distribution
  • Zebrafish/physiology*
PubMed
17531198 Full text @ Biochem. Biophys. Res. Commun.
Abstract
The basic helix-loop-helix (bHLH) protein Mist1 is an important exocrine pancreas transcriptional factor expressed in the acinar cells of mammals. In the present study, we cloned the homologous Mist1 cDNA encoding a predicted protein of 184 amino acids in zebrafish. The typical bHLH domain of zebrafish Mist1 shares high identity with that of its orthologs in mouse, rat, and human. Expression analysis revealed that Mist1 maternal transcripts are distinct in the very beginning of embryogenesis and that endogenous Mist1 is chronologically expressed in polster, hatching gland, hindbrain and appears exclusively in the pancreas from 72hpf onward. Knockdown of Mist1 conditionally causes mild morphological defects in embryos. In MO-treated embryos, midbrain-hindbrain boundary is missing and exocrine pancreas is significantly reduced and disorganized. These results suggest that Mist1 functions in an evolutionary conserved way as a key transcriptional regulator specific for exocrine pancreas development in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping