PUBLICATION
Two inducible, functional cyclooxygenase-2 genes are present in the rainbow trout genome
- Authors
- Ishikawa, T.O., and Herschman, H.R.
- ID
- ZDB-PUB-070523-32
- Date
- 2007
- Source
- Journal of cellular biochemistry 102(6): 1486-1492 (Journal)
- Registered Authors
- Keywords
- Oncorhynchus mykiss, prostaglandins, gene duplication, Cox-2, teleost genome
- MeSH Terms
-
- 3' Untranslated Regions
- Amino Acid Sequence
- Animals
- Base Sequence
- Binding Sites
- COS Cells
- Cell Culture Techniques
- Cells, Cultured
- Chlorocebus aethiops
- Cloning, Molecular
- Conserved Sequence
- Cyclooxygenase 2/biosynthesis*
- Cyclooxygenase 2/chemistry
- Cyclooxygenase 2/genetics*
- Cyclooxygenase 2/metabolism
- DNA, Complementary/genetics
- Enzyme Induction*
- Exons
- Genetic Variation
- Genome*
- Introns
- Isoenzymes/biosynthesis
- Isoenzymes/chemistry
- Isoenzymes/genetics
- Isoenzymes/metabolism
- Molecular Sequence Data
- Oncorhynchus mykiss/genetics*
- Open Reading Frames
- Protein Binding
- Protein Conformation
- Protein Structure, Tertiary
- RNA, Messenger/metabolism
- Sequence Homology, Amino Acid
- Substrate Specificity
- PubMed
- 17471498 Full text @ J. Cell. Biochem.
Citation
Ishikawa, T.O., and Herschman, H.R. (2007) Two inducible, functional cyclooxygenase-2 genes are present in the rainbow trout genome. Journal of cellular biochemistry. 102(6):1486-1492.
Abstract
The cyclooxygenases (Cox) catalyze the initial reactions in prostanoid biosynthesis, and produce the common prostanoids precursor, PGH(2). Mammalian species have two Cox isoforms; constitutively expressed cyclooxygenase-1 (Cox-1) and inducible cyclooxygenase-2 (Cox-2). Database searches suggest three Cox genes are present in many fish species. In this study, we cloned and characterized a second Cox-2 cDNA, Cox-2b, from the rainbow trout. Rainbow trout Cox-2b protein contains all the functionally important conserved amino acids for Cox enzyme activity. Moreover, the Cox-2b message contains AU-rich elements (AREs) in the 3' untranslated region (3'UTR) characteristic of inducible Cox-2 mRNAs. We took advantage of the existence of a rainbow trout cell line to demonstrate that expression from both the originally reported Cox-2 (Cox-2a) and Cox-2b genes is inducible. However, differential induction responses to alternative inducers are observed for rainbow trout Cox-2a and Cox-2b. Both Cox-2a and Cox-2b proteins expressed in COS cells are enzymatically active. Thus the rainbow trout has two functional, inducible Cox-2 genes. The zebrafish also contains two Cox-2 genes. However, genome structure analysis suggests diversion of the Cox-2a gene between zebrafish and rainbow trout.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping