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ZFIN ID: ZDB-PUB-070523-32
Two inducible, functional cyclooxygenase-2 genes are present in the rainbow trout genome
Ishikawa, T.O., and Herschman, H.R.
Date: 2007
Source: Journal of cellular biochemistry 102(6): 1486-1492 (Journal)
Registered Authors:
Keywords: Oncorhynchus mykiss, prostaglandins, gene duplication, Cox-2, teleost genome
MeSH Terms:
  • 3' Untranslated Regions
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Binding Sites
  • COS Cells
  • Cell Culture Techniques
  • Cells, Cultured
  • Cercopithecus aethiops
  • Cloning, Molecular
  • Conserved Sequence
  • Cyclooxygenase 2/biosynthesis*
  • Cyclooxygenase 2/chemistry
  • Cyclooxygenase 2/genetics*
  • Cyclooxygenase 2/metabolism
  • DNA, Complementary/genetics
  • Enzyme Induction*
  • Exons
  • Genetic Variation
  • Genome*
  • Introns
  • Isoenzymes/biosynthesis
  • Isoenzymes/chemistry
  • Isoenzymes/genetics
  • Isoenzymes/metabolism
  • Molecular Sequence Data
  • Oncorhynchus mykiss/genetics*
  • Open Reading Frames
  • Protein Binding
  • Protein Conformation
  • Protein Structure, Tertiary
  • RNA, Messenger/metabolism
  • Sequence Homology, Amino Acid
  • Substrate Specificity
PubMed: 17471498 Full text @ J. Cell. Biochem.
The cyclooxygenases (Cox) catalyze the initial reactions in prostanoid biosynthesis, and produce the common prostanoids precursor, PGH(2). Mammalian species have two Cox isoforms; constitutively expressed cyclooxygenase-1 (Cox-1) and inducible cyclooxygenase-2 (Cox-2). Database searches suggest three Cox genes are present in many fish species. In this study, we cloned and characterized a second Cox-2 cDNA, Cox-2b, from the rainbow trout. Rainbow trout Cox-2b protein contains all the functionally important conserved amino acids for Cox enzyme activity. Moreover, the Cox-2b message contains AU-rich elements (AREs) in the 3' untranslated region (3'UTR) characteristic of inducible Cox-2 mRNAs. We took advantage of the existence of a rainbow trout cell line to demonstrate that expression from both the originally reported Cox-2 (Cox-2a) and Cox-2b genes is inducible. However, differential induction responses to alternative inducers are observed for rainbow trout Cox-2a and Cox-2b. Both Cox-2a and Cox-2b proteins expressed in COS cells are enzymatically active. Thus the rainbow trout has two functional, inducible Cox-2 genes. The zebrafish also contains two Cox-2 genes. However, genome structure analysis suggests diversion of the Cox-2a gene between zebrafish and rainbow trout.