ZFIN ID: ZDB-PUB-070513-19
Zebrafish spata2 is expressed at early developmental stages
Moro, E., Maran, C., Slongo, M.L., Argenton, F., Toppo, S., and Onisto, M.
Date: 2007
Source: The International journal of developmental biology   51(3): 241-246 (Journal)
Registered Authors: Argenton, Francesco, Moro, Enrico
Keywords: none
MeSH Terms:
  • 3' Untranslated Regions
  • 5' Untranslated Regions
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • Codon, Terminator
  • Computational Biology
  • Consensus Sequence
  • DNA, Complementary
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental*
  • In Situ Hybridization
  • Male
  • Molecular Sequence Data
  • Open Reading Frames
  • Organ Specificity
  • Polymerase Chain Reaction
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Proteins
  • RNA, Messenger
  • Sequence Homology, Amino Acid
  • Spermatogenesis/genetics
  • Testis/metabolism
  • Tissue Distribution
  • Zebrafish/embryology*
  • Zebrafish Proteins/chemistry
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 17486545 Full text @ Int. J. Dev. Biol.
Spata2 (spermatogenesis-associated protein 2) was originally described as a novel gene involved in the spermatogenic process. In this study, we cloned a potential zebrafish spata2 orthologue. The consensus open reading frame (1650 bp) encodes a polypeptide of 550 amino acids which shares 37% identity with the human SPATA2. Bioinformatic analysis reveals a small pattern PW [KR] KE [YF][RK] which seems to be of particular interest in the light of its strong conservation between SPATA2 and the recently discovered TAMO protein of D. melanogaster. RT-PCR analysis in adult zebrafish tissues revealed that spata2 mRNA has a broad distribution. Whole-mount in situ hybridization demonstrated that spata2 transcripts are maternally derived and becomes strongly localized in the central nervous system at early developmental stages. From 5 dpf, spata2 expression becomes detectable in the gut and pronephric duct epithelium, suggesting a wide tissue function during vertebrate development.