PUBLICATION
Zebrafish spata2 is expressed at early developmental stages
- Authors
- Moro, E., Maran, C., Slongo, M.L., Argenton, F., Toppo, S., and Onisto, M.
- ID
- ZDB-PUB-070513-19
- Date
- 2007
- Source
- The International journal of developmental biology 51(3): 241-246 (Journal)
- Registered Authors
- Argenton, Francesco, Moro, Enrico
- Keywords
- none
- MeSH Terms
-
- 3' Untranslated Regions
- 5' Untranslated Regions
- Amino Acid Sequence
- Animals
- Base Sequence
- Cloning, Molecular
- Codon, Terminator
- Computational Biology
- Consensus Sequence
- DNA, Complementary
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental*
- In Situ Hybridization
- Male
- Molecular Sequence Data
- Open Reading Frames
- Organ Specificity
- Polymerase Chain Reaction
- Protein Structure, Secondary
- Protein Structure, Tertiary
- Proteins
- RNA, Messenger
- Sequence Homology, Amino Acid
- Spermatogenesis/genetics
- Testis/metabolism
- Tissue Distribution
- Zebrafish/embryology*
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 17486545 Full text @ Int. J. Dev. Biol.
Citation
Moro, E., Maran, C., Slongo, M.L., Argenton, F., Toppo, S., and Onisto, M. (2007) Zebrafish spata2 is expressed at early developmental stages. The International journal of developmental biology. 51(3):241-246.
Abstract
Spata2 (spermatogenesis-associated protein 2) was originally described as a novel gene involved in the spermatogenic process. In this study, we cloned a potential zebrafish spata2 orthologue. The consensus open reading frame (1650 bp) encodes a polypeptide of 550 amino acids which shares 37% identity with the human SPATA2. Bioinformatic analysis reveals a small pattern PW [KR] KE [YF][RK] which seems to be of particular interest in the light of its strong conservation between SPATA2 and the recently discovered TAMO protein of D. melanogaster. RT-PCR analysis in adult zebrafish tissues revealed that spata2 mRNA has a broad distribution. Whole-mount in situ hybridization demonstrated that spata2 transcripts are maternally derived and becomes strongly localized in the central nervous system at early developmental stages. From 5 dpf, spata2 expression becomes detectable in the gut and pronephric duct epithelium, suggesting a wide tissue function during vertebrate development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping