|ZFIN ID: ZDB-PUB-070513-19|
Zebrafish spata2 is expressed at early developmental stages
Moro, E., Maran, C., Slongo, M.L., Argenton, F., Toppo, S., and Onisto, M.
|Source:||The International journal of developmental biology 51(3): 241-246 (Journal)|
|Registered Authors:||Argenton, Francesco, Moro, Enrico|
|PubMed:||17486545 Full text @ Int. J. Dev. Biol.|
Moro, E., Maran, C., Slongo, M.L., Argenton, F., Toppo, S., and Onisto, M. (2007) Zebrafish spata2 is expressed at early developmental stages. The International journal of developmental biology. 51(3):241-246.
ABSTRACTSpata2 (spermatogenesis-associated protein 2) was originally described as a novel gene involved in the spermatogenic process. In this study, we cloned a potential zebrafish spata2 orthologue. The consensus open reading frame (1650 bp) encodes a polypeptide of 550 amino acids which shares 37% identity with the human SPATA2. Bioinformatic analysis reveals a small pattern PW [KR] KE [YF][RK] which seems to be of particular interest in the light of its strong conservation between SPATA2 and the recently discovered TAMO protein of D. melanogaster. RT-PCR analysis in adult zebrafish tissues revealed that spata2 mRNA has a broad distribution. Whole-mount in situ hybridization demonstrated that spata2 transcripts are maternally derived and becomes strongly localized in the central nervous system at early developmental stages. From 5 dpf, spata2 expression becomes detectable in the gut and pronephric duct epithelium, suggesting a wide tissue function during vertebrate development.