PUBLICATION

A critical role of vascular endothelial growth factor D in zebrafish embryonic vasculogenesis and angiogenesis

Authors
Song, M., Yang, H., Yao, S., Ma, F., Li, Z., Deng, Y., Deng, H., Zhou, Q., Shuo, L., and Wei, Y.
ID
ZDB-PUB-070504-21
Date
2007
Source
Biochemical and Biophysical Research Communications   357(4): 924-930 (Journal)
Registered Authors
Li, Zheng
Keywords
VEGF-D, Zebrafish, Development, Vasculogenesis, Angiogenesis
MeSH Terms
  • Animals
  • Blood Vessels/growth & development*
  • Embryonic Development/physiology*
  • Gene Expression Regulation, Developmental/physiology
  • Neovascularization, Physiologic/physiology*
  • Vascular Endothelial Growth Factor D/metabolism*
  • Zebrafish/embryology*
  • Zebrafish/physiology*
PubMed
17466267 Full text @ Biochem. Biophys. Res. Commun.
Abstract
The VEGF family comprises seven members that are designated VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, placental growth factor (PlGF), and VEGF-F. Of these factors, VEGF-D plays important roles for angiogenesis and lymphangiogenesis, and could promote tumor growth and lymphatic metastasis. In this study, we identified a zebrafish VEGF-D homolog that encodes a 272 amino acid protein including a PDGF (platelet-derived growth factor) domain characteristic to VEGF family. Expression profile demonstrated that the VEGF-D began expressed from 13 somite stage. Microinjecting zVEGF-D mRNA into zebrafish 1-cell stage embryos resulted in severe misguidance of intersegmental vessels (ISV) and abnormal connection between dorsal aorta and caudal vein. Microangiography indicated that these abnormal ISVs were not functional. Our studies therefore identified the first non-mammalian VEGF-D and established its in vivo role for vascular system development during vertebrate embryogenesis and provided an alternative animal model to further reveal functions of VEGF-D.
Genes / Markers
Figures
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping