PUBLICATION
In vivo imaging and genetic analysis link bacterial motility and symbiosis in the zebrafish gut
- Authors
- Rawls, J.F., Mahowald, M.A., Goodman, A.L., Trent, C.M., and Gordon, J.I.
- ID
- ZDB-PUB-070504-16
- Date
- 2007
- Source
- Proceedings of the National Academy of Sciences of the United States of America 104(18): 7622-7627 (Journal)
- Registered Authors
- Gordon, Jeffrey I., Mahowald, Michael A., Rawls, John F., Trent, Chad
- Keywords
- Danio rerio, establishment of a gut microbiota, flagellar motility, host?microbial symbiosis and mutualism, Pseudomonas aeruginosa
- MeSH Terms
-
- Flagella/genetics
- Flagella/physiology
- Time Factors
- Zebrafish/immunology
- Zebrafish/microbiology*
- Microscopy, Electron, Transmission
- Intestines/immunology
- Intestines/microbiology*
- Intestines/ultrastructure
- Pseudomonas aeruginosa/genetics*
- Pseudomonas aeruginosa/immunology
- Pseudomonas aeruginosa/physiology*
- Pseudomonas aeruginosa/ultrastructure
- Animals
- Movement*
- Germ-Free Life
- Symbiosis*/genetics
- PubMed
- 17456593 Full text @ Proc. Natl. Acad. Sci. USA
Citation
Rawls, J.F., Mahowald, M.A., Goodman, A.L., Trent, C.M., and Gordon, J.I. (2007) In vivo imaging and genetic analysis link bacterial motility and symbiosis in the zebrafish gut. Proceedings of the National Academy of Sciences of the United States of America. 104(18):7622-7627.
Abstract
Complex microbial communities reside within the intestines of humans and other vertebrates. Remarkably little is known about how these microbial consortia are established in various locations within the gut, how members of these consortia behave within their dynamic ecosystems, or what microbial factors mediate mutually beneficial host-microbial interactions. Using a gnotobiotic zebrafish-Pseudomonas aeruginosa model, we show that the transparency of this vertebrate species, coupled with methods for raising these animals under germ-free conditions can be used to monitor microbial movement and localization within the intestine in vivo and in real time. Germ-free zebrafish colonized with isogenic P. aeruginosa strains containing deletions of genes related to motility and pathogenesis revealed that loss of flagellar function results in attenuation of evolutionarily conserved host innate immune responses but not conserved nutrient responses. These results demonstrate the utility of gnotobiotic zebrafish in defining the behavior and localization of bacteria within the living vertebrate gut, identifying bacterial genes that affect these processes, and assessing the impact of these genes on host-microbial interactions.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping