PUBLICATION
A tolloid homologue from the Pacific oyster Crassostrea gigas
- Authors
- Herpin, A., Lelong, C., Becker, T., Favrel, P., and Cunningham, C.
- ID
- ZDB-PUB-070427-15
- Date
- 2007
- Source
- Gene expression patterns : GEP 7(6): 700-708 (Journal)
- Registered Authors
- Becker, Thomas S.
- Keywords
- Tolloid, Crassostrea gigas, Oyster, Bivalve, Zebrafish, Lophotrochozoa, Ecdysozoa, Mollusc, Bone morphogenetic protein, Transforming growth factor β, Mesoderm patterning, Embryogenesis
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Drosophila melanogaster/enzymology
- Drosophila melanogaster/genetics
- Drosophila melanogaster/growth & development
- Embryo, Nonmammalian/physiology
- Exons
- Gene Expression Regulation, Developmental
- Introns
- Larva/physiology
- Mammals/genetics
- Molecular Sequence Data
- Ostreidae/embryology
- Ostreidae/enzymology
- Ostreidae/genetics*
- Ostreidae/growth & development
- Phylogeny
- Reverse Transcriptase Polymerase Chain Reaction
- Tolloid-Like Metalloproteinases/chemistry
- Tolloid-Like Metalloproteinases/genetics*
- Transforming Growth Factor beta/genetics
- Zebrafish/genetics
- PubMed
- 17433792 Full text @ Gene Expr. Patterns
Citation
Herpin, A., Lelong, C., Becker, T., Favrel, P., and Cunningham, C. (2007) A tolloid homologue from the Pacific oyster Crassostrea gigas. Gene expression patterns : GEP. 7(6):700-708.
Abstract
The genes governing mesoderm specification have been extensively studied in vertebrates, arthropods and nematodes. The latter two phyla belong to the Ecdysozoan clade but little is understood of the role that these genes might play in the development of the other major protostomal clade, the Lophotrochozoa. As part of a wider project to analyze the functions associated with transforming growth factor beta superfamily members in Lophotrochozoa, we have cloned a gene encoding a tolloid homologue from the bivalve mollusc Crassostrea gigas. Tolloid is a key developmental protein that regulates the activity of bone morphogenetic proteins (BMPs). We have determined the intron-exon structure of the gene encoding C. gigas tolloid and have compared it with those of homologous genes from both protostomes and deuterostomes. In order to analyze the functionality of oyster tolloid the zebrafish embryo has been employed as a reporter organism and we show that over-expression of this protein results in the ventralization of zebrafish embryos at 24h post fertilization. The expression of the C. gigas tolloid gene during embryonic and larval development as well as in adult tissues is also explored.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping