PUBLICATION
A major zebrafish polymorphism resource for genetic mapping
- Authors
- Bradley, K.M., Elmore, J.B., Breyer, J.P., Yaspan, B.L., Jessen, J.R., Knapik, E.W., and Smith, J.R.
- ID
- ZDB-PUB-070427-10
- Date
- 2007
- Source
- Genome biology 8(4): R55 (Journal)
- Registered Authors
- Jessen, Jason R., Knapik, Ela W.
- Keywords
- none
- MeSH Terms
-
- Animals
- Chromosome Mapping/methods*
- Cloning, Molecular
- Genetic Linkage
- Genetic Markers
- Polymorphism, Single Nucleotide*
- Zebrafish/genetics*
- PubMed
- 17428331 Full text @ Genome Biol.
Citation
Bradley, K.M., Elmore, J.B., Breyer, J.P., Yaspan, B.L., Jessen, J.R., Knapik, E.W., and Smith, J.R. (2007) A major zebrafish polymorphism resource for genetic mapping. Genome biology. 8(4):R55.
Abstract
We identify 645,088 candidate polymorphisms in zebrafish and observe a single nucleotide polymorphism (SNP) validation rate of 71% - 86%, improving with polymorphism confidence score. Variant sites are non-random, with an excess of specific novel T- and A-rich motifs. We position half of the polymorphisms on zebrafish genetic and physical maps as a resource for positional cloning. We further demonstrate bulked segregant analysis using the anchored SNPs as a method for high-throughput genetic mapping in zebrafish.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping