PUBLICATION
Priming, initiation and synchronization of the segmentation clock by deltaD and deltaC
- Authors
- Mara, A., Schroeder, J., Chalouni, C., and Holley, S.A.
- ID
- ZDB-PUB-070413-5
- Date
- 2007
- Source
- Nature cell biology 9(5): 523-530 (Journal)
- Registered Authors
- Holley, Scott
- Keywords
- none
- MeSH Terms
-
- Amyloid Precursor Protein Secretases/antagonists & inhibitors
- Amyloid Precursor Protein Secretases/metabolism
- Somites/drug effects
- Somites/metabolism*
- Embryonic Stem Cells/metabolism
- Enzyme Inhibitors/pharmacology
- Biological Clocks*/drug effects
- Basic Helix-Loop-Helix Transcription Factors/metabolism
- Membrane Proteins/genetics
- Membrane Proteins/metabolism*
- Transcription Factors/metabolism
- Time Factors
- Animals
- Intracellular Signaling Peptides and Proteins
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism*
- Cell Movement
- Signal Transduction
- Microinjections
- Mutation
- Gene Expression Regulation, Developmental*/drug effects
- Zebrafish/embryology*
- Zebrafish/metabolism
- Receptors, Notch/metabolism
- RNA, Messenger/metabolism
- In Situ Hybridization
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Tissue Culture Techniques
- Dipeptides/pharmacology
- PubMed
- 17417625 Full text @ Nat. Cell Biol.
Citation
Mara, A., Schroeder, J., Chalouni, C., and Holley, S.A. (2007) Priming, initiation and synchronization of the segmentation clock by deltaD and deltaC. Nature cell biology. 9(5):523-530.
Abstract
Zebrafish somitogenesis is governed by a segmentation clock that generates oscillations in expression of several Notch pathway genes, including her1, her7 and deltaC. Using a combination of pharmacological inhibition and Mendelian genetics, we show that DeltaD and DeltaC, two Notch ligands, represent functionally distinct signals within the segmentation clock. Using high-resolution fluorescent in situ hybridization, the oscillations were divided into phases based on eight distinct subcellular patterns of mRNA localization for 140,000 cells. her1, her7 and deltaC expression was examined in wild-type, deltaD(-/-) and deltaC(-/-) embryos. We identified areas within the tailbud where the clock is set up in the progenitor cells (priming), where the clock starts running (initiation), and where the clocks of neighbouring cells are entrained (synchronization). We find that the clocks of motile cells are primed by deltaD in a progenitor zone in the posterior tailbud and that deltaD is required for cells to initiate oscillations on exiting this zone. Oscillations of adjacent cells are synchronized and amplified by deltaC in the posterior presomitic mesoderm as cell movement subsides and cells maintain stable neighbour relationships.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping