PUBLICATION
Testing estrogenicity of known and novel (xeno-)estrogens in the MolDarT using developing zebrafish (Danio rerio)
- Authors
- Muncke, J., Junghans, M., and Eggen, R.I.
- ID
- ZDB-PUB-070330-15
- Date
- 2007
- Source
- Environmental toxicology 22(2): 185-193 (Journal)
- Registered Authors
- Keywords
- atrazine, cyproconazol, estradiol, ethinylestradiol, nonylphenol, developing zebrafish, MolDarT, ecotoxicology, YES, real-time PCR
- MeSH Terms
-
- Animals
- Atrazine/toxicity
- Benzhydryl Compounds
- Biological Assay
- Estrogens/toxicity*
- Ethinyl Estradiol/toxicity
- Fungicides, Industrial/toxicity
- Herbicides/toxicity
- Phenols/toxicity
- Receptors, Estrogen/metabolism
- Triazoles/toxicity
- Vitellogenins/metabolism*
- Zebrafish/embryology
- Zebrafish/metabolism*
- PubMed
- 17366571 Full text @ Env. Tox.
- CTD
- 17366571
Citation
Muncke, J., Junghans, M., and Eggen, R.I. (2007) Testing estrogenicity of known and novel (xeno-)estrogens in the MolDarT using developing zebrafish (Danio rerio). Environmental toxicology. 22(2):185-193.
Abstract
The MolDarT is a novel short-term assay for testing mechanism-based molecular effects in developing zebrafish embryos. The objective of this study was to evaluate the inducibility of vitellogenin1 mRNA (Vtg1) by the estrogenically active compounds 17beta-Estradiol (E2), 17alpha-Ethinylestradiol (EE2), Nonylphenol (NP), Bisphenol A (BPA), Cyproconazol, and the suspected xeno-estrogen Atrazin in the MolDarT. Freshly fertilized zebrafish eggs were exposed semistatically for 120 h. Using reverse transcription real-time PCR, the relative abundance of Vtg1 was measured. For EE2 a dose-response relationship was established with EC50 = 60.7 ng/L (205 pM). Induction of Vtg1 was significant at concentrations of 84 pM EE2 (25 ng EE2/L) and above, 10 nM E2 (2.7 mug E2/L), 100 nM E2 (27 mug E2/L), 10 muM BPA (2280 mug BPA/L), and 15 muM BPA (3420 mug BPA/L). At NP concentrations of 0.75 muM (165 mug NP/L) and 1.5 muM (330 mug NP/L) Vtg1 was significantly down-regulated. Both atrazine and cyproconazol showed no effect on relative Vtg1 abundance. With this study we further characterize the MolDarT assay and show its applicability for effect screening of compounds.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping