PUBLICATION
Using Morpholinos to Control Gene Expression
- Authors
- Moulton, J.D.
- ID
- ZDB-PUB-070307-1
- Date
- 2007
- Source
- Current Protocols in Nucleic Acid Chemistry Suppl 27: Unit 4.30 (Chapter)
- Registered Authors
- Moulton, Jon D.
- Keywords
- Morpholino, antisense, oligo, knockdown, splicing
- MeSH Terms
- none
- PubMed
- none
Citation
Moulton, J.D. (2007) Using Morpholinos to Control Gene Expression. Current Protocols in Nucleic Acid Chemistry. Suppl 27:Unit 4.30.
Abstract
Morpholino oligonucleotides are stable, uncharged, water-soluble molecules used to block complementary sequences of RNA, preventing processing, read-through, or protein binding at those sites. Morpholinos are typically used to block translation of mRNA and to block splicing of pre-mRNA, though they can block other interactions between biological macromolecules and RNA. Morpholinos are effective, specific, and lack non-antisense effects. They work in any cell that transcribes and translates RNA, but must be delivered into the nuclear/cytosolic compartment to be effective. Morpholinos form stable base pairs with complementary nucleic acid sequences but apparently do not bind to proteins to a significant extent. They are not recognized by any proteins and do not undergo protein-mediated catalysis; nor do they mediate RNA cleavage by RNase H or the RISC complex. This work focuses on techniques and background for using Morpholinos.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping