PUBLICATION

Cloning of zebrafish nkx6.2 and a comprehensive analysis of the conserved transcriptional response to Hedgehog/Gli signaling in the zebrafish neural tube

Authors
Guner, B., and Karlstrom, R.O.
ID
ZDB-PUB-070303-24
Date
2007
Source
Gene expression patterns : GEP   7(5): 596-605 (Journal)
Registered Authors
Guner-Ataman, Burcu, Karlstrom, Rolf
Keywords
dbx1a, dbx2, hlx1, hlxb9, irx1a, irx3a, irx5, nkx2.2a, nkx2.9, nkx6.1, nkx6.2, pax3, pax6a, pax7, Chameleon, Detour, Slow muscle omitted, You-too, Cyclopamine, Floorplate, Motor neurons
MeSH Terms
  • Zebrafish/embryology
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Gene Expression Regulation, Developmental*
  • Hedgehog Proteins/metabolism*
  • Animals
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism*
  • Transcription Factors/genetics*
  • Transcription Factors/metabolism*
  • DNA, Complementary/genetics
  • DNA, Complementary/metabolism
  • Embryo, Nonmammalian/cytology
  • Embryo, Nonmammalian/metabolism
  • RNA, Messenger/administration & dosage
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Transcription, Genetic*
  • Signal Transduction
  • Cloning, Molecular
  • Neurons/cytology
  • Neurons/metabolism*
  • In Situ Hybridization
  • RNA Probes
(all 24)
PubMed
17307034 Full text @ Gene Expr. Patterns
Abstract
Sonic Hedgehog (Shh) signaling helps pattern the vertebrate neural tube, in part by regulating the dorsal/ventral expression of a number of homeodomain containing transcription factors. These Hh responsive genes have been divided into two classes, with Class II genes being activated by Hh signaling and Class I genes being repressed by Hh signaling. While the transcriptional response to varying Hh levels is well defined in chick and mouse, it is only partially described in zebrafish, despite the fact that zebrafish has emerged as a powerful genetic system for the study of neural patterning. To better characterize the Hh response in the zebrafish neural tube, we cloned the zebrafish Class II Hh target genes nkx2.9 and nkx6.2. We then analyzed the expression of a number of Class I and Class II Hh responsive genes in wild type, Hh mutant, and Hh over-expressing zebrafish embryos. We show that expression of Class I and Class II genes is highly conserved in the vertebrate neural tube. Further, ventral-most Class II gene expression was completely lost in all Hh pathway mutants analyzed, indicating high levels of Hh signaling are blocked in all of these mutants. In contrast, more dorsally expressed genes were variably affected in different Hh pathway mutants, indicating mid-levels of Hh signaling are differentially affected. This comprehensive expression study provides an important tool for the characterization of Hh signaling in zebrafish and provides a sensitive assay for determining the degree to which newly identified zebrafish mutants affect Hh signaling.
Genes / Markers
Figures
Figure Gallery (2 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b641
    		Point Mutation
    tm15a
      		Point Mutation
      ts269
        		Point Mutation
        ty17a
          		Point Mutation
          1 - 4 of 4
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          Human Disease / Model
          No data available
          Sequence Targeting Reagents
          No data available
          Fish
          Fish
          disp1tm15a/tm15a
          gli1ts269/ts269
          gli2aty17a/ty17a
          smob641/b641 (AB)
          WT
          1 - 5 of 5
          Show
          Antibodies
          No data available
          Orthology
          Gene Orthology
          nkx6.2
          1 - 1 of 1
          Show
          Engineered Foreign Genes
          No data available
          Mapping
          No data available
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          Citation
          Guner, B., and Karlstrom, R.O. (2007) Cloning of zebrafish nkx6.2 and a comprehensive analysis of the conserved transcriptional response to Hedgehog/Gli signaling in the zebrafish neural tube. Gene expression patterns : GEP. 7(5):596-605.