ZFIN ID: ZDB-PUB-070303-22
Expression of the G protein gammaT1 subunit during zebrafish development
Chen, H., Leung, T., Giger, K.E., Stauffer, A.M., Humbert, J.E., Sinha, S., Horstick, E.J., Hansen, C.A., and Robishaw, J.D.
Date: 2007
Source: Gene expression patterns : GEP   7(5): 574-583 (Journal)
Registered Authors: Chen, Hui, Humbert, Jasper, Leung, Tin Chung, Sinha, Soniya
Keywords: Zebrafish, G protein, gngT1, Signal transduction, Rhodopsin, Exo-rhodopsin, Pineal gland, Retina, Photoreceptor cells
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cloning, Molecular
  • GTP-Binding Protein gamma Subunits/genetics
  • GTP-Binding Protein gamma Subunits/metabolism*
  • Gene Expression Regulation, Developmental*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • In Situ Hybridization
  • Molecular Sequence Data
  • Oligonucleotides, Antisense/pharmacology
  • Otx Transcription Factors/genetics
  • Otx Transcription Factors/metabolism
  • Photoreceptor Cells/metabolism
  • Pineal Gland/cytology
  • Pineal Gland/metabolism
  • RNA Probes
  • Retina/cytology
  • Retina/metabolism
  • Rhodopsin/metabolism
  • Sequence Homology, Amino Acid
  • Trans-Activators/genetics
  • Trans-Activators/metabolism
  • Zebrafish/genetics*
  • Zebrafish/growth & development
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism*
PubMed: 17306630 Full text @ Gene Expr. Patterns
Here, we report the identification and expression analysis of the zebrafish G protein gammaT1 subunit gene (gngT1) during development. Similar to its human and mouse homologs, we confirm zebrafish gngT1 is expressed in the developing retina, where its transcription overlaps with the photoreceptor cell-specific marker, rhodopsin (rho). Surprisingly, we also show zebrafish gngT1 is expressed in the dorsal diencephalon, where its transcription overlaps with the pineal specific markers, arylalkylamine N-acetyltransferase-2 (annat-2) and extra-ocular rhodopsin (exorh). Analysis of the proximal promoter sequence of the zebrafish gngT1 gene identifies several conserved binding sites for the cone-rod homeobox/orthodenticle (Crx/Otx) homeodomain family of transcription factors. Using a morpholino anti-sense approach in zebrafish, we show that targeted knockdown of otx5 potently suppresses gngT1 expression in the pineal gland, whereas knockdown of crx markedly reduces gngT1 expression in the retina. Taken together, these data indicate that pineal- and retinal-specific expression of the gngT1 gene are controlled by different transcription factors and exogenous signals.