|ZFIN ID: ZDB-PUB-070303-22|
Expression of the G protein gammaT1 subunit during zebrafish development
Chen, H., Leung, T., Giger, K.E., Stauffer, A.M., Humbert, J.E., Sinha, S., Horstick, E.J., Hansen, C.A., and Robishaw, J.D.
|Source:||Gene expression patterns : GEP 7(5): 574-583 (Journal)|
|Registered Authors:||Chen, Hui, Humbert, Jasper, Leung, Tin Chung, Sinha, Soniya|
|Keywords:||Zebrafish, G protein, gngT1, Signal transduction, Rhodopsin, Exo-rhodopsin, Pineal gland, Retina, Photoreceptor cells|
|PubMed:||17306630 Full text @ Gene Expr. Patterns|
Chen, H., Leung, T., Giger, K.E., Stauffer, A.M., Humbert, J.E., Sinha, S., Horstick, E.J., Hansen, C.A., and Robishaw, J.D. (2007) Expression of the G protein gammaT1 subunit during zebrafish development. Gene expression patterns : GEP. 7(5):574-583.
ABSTRACTHere, we report the identification and expression analysis of the zebrafish G protein gammaT1 subunit gene (gngT1) during development. Similar to its human and mouse homologs, we confirm zebrafish gngT1 is expressed in the developing retina, where its transcription overlaps with the photoreceptor cell-specific marker, rhodopsin (rho). Surprisingly, we also show zebrafish gngT1 is expressed in the dorsal diencephalon, where its transcription overlaps with the pineal specific markers, arylalkylamine N-acetyltransferase-2 (annat-2) and extra-ocular rhodopsin (exorh). Analysis of the proximal promoter sequence of the zebrafish gngT1 gene identifies several conserved binding sites for the cone-rod homeobox/orthodenticle (Crx/Otx) homeodomain family of transcription factors. Using a morpholino anti-sense approach in zebrafish, we show that targeted knockdown of otx5 potently suppresses gngT1 expression in the pineal gland, whereas knockdown of crx markedly reduces gngT1 expression in the retina. Taken together, these data indicate that pineal- and retinal-specific expression of the gngT1 gene are controlled by different transcription factors and exogenous signals.