PUBLICATION

Comparative analysis of splice form-specific expression of LIM Kinases during zebrafish development

Authors
Ott, E.B., Te Velthuis, A.J., and Bagowski, C.P.
ID
ZDB-PUB-070303-14
Date
2007
Source
Gene expression patterns : GEP   7(5): 620-629 (Journal)
Registered Authors
Bagowski, Christoph P., Ott, Elisabeth B.
Keywords
LIM domain kinase 1, LIM domain kinase 2, LIMK1, LIMK2, PDZ, LIM, Gene expression, In situ hybridization, Embryogenesis, Development, Zebrafish
MeSH Terms
  • Alternative Splicing*
  • Animals
  • Binding Sites
  • Cloning, Molecular
  • Gene Expression Regulation, Developmental*
  • Lim Kinases
  • Protein Kinases/genetics*
  • Protein Kinases/metabolism
  • Regulatory Elements, Transcriptional
  • Zebrafish/embryology
  • Zebrafish/genetics*
  • Zebrafish/metabolism
PubMed
17300993 Full text @ Gene Expr. Patterns
Abstract
LIM Kinases (LIMK) are genes encoding multi-domain proteins that can contain up to two LIM domains, a single PDZ domain, and a tyrosine kinase domain. Alternative splicing is a source for different combinations of these domains. Two family members, LIMK1 and LIMK2 have been described in mammals and are important for organization of the actin cytoskeleton. We have cloned LIMK1 and LIMK2 from zebrafish and characterized their domain specific expression patterns during embryogenesis. The results on temporal and spatial expression of the LIM Kinases during embryogenesis indicate overlapping and distinct expression domains for LMK1 and LIMK2. Differences in expression during embryogenesis were observed for PDZ and LIM encoding splice forms for both LIM Kinases. To better understand the transcriptional regulation of LIM Kinases, we searched for conserved regulatory elements. We identified evolutionary conserved smad binding sites for LIMK2. In summary, we present here the splice-form specific temporal and spatial expression patterns for both LIMK1 and LIMK2 during zebrafish embryogenesis.
Genes / Markers
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Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping