PUBLICATION
Eye field requires the function of Sfrp1 as a Wnt antagonist
- Authors
- Kim, H.S., Shin, J., Kim, S.H., Chun, H.S., Kim, J.D., Kim, Y.S., Kim, M.J., Rhee, M., Yeo, S.Y., and Huh, T.L.
- ID
- ZDB-PUB-070210-3
- Date
- 2007
- Source
- Neuroscience letters 414(1): 26-29 (Journal)
- Registered Authors
- Huh, Tae-Lin, Kim, Hyung-Seok, Kim, Seok-Hyung, Shin, Jimann, Yeo, Sang-Yeob
- Keywords
- sfrp1, Wnt8b, Zebrafish, Wnt antagonist, Eye, Brain patterning
- MeSH Terms
-
- Cytoskeletal Proteins/genetics
- Cytoskeletal Proteins/metabolism
- Signal Transduction/genetics*
- Organogenesis/genetics
- Visual Pathways/cytology
- Visual Pathways/embryology*
- Visual Pathways/metabolism
- Intercellular Signaling Peptides and Proteins/genetics
- Mesencephalon/cytology
- Mesencephalon/embryology
- Mesencephalon/metabolism
- Eye/cytology
- Eye/embryology
- Eye/metabolism
- Animals
- Prosencephalon/cytology
- Prosencephalon/embryology*
- Prosencephalon/metabolism
- PAX2 Transcription Factor/genetics
- PAX2 Transcription Factor/metabolism
- Nervous System Malformations/genetics
- Nervous System Malformations/metabolism
- Nervous System Malformations/physiopathology
- Wnt Proteins/genetics*
- Wnt Proteins/metabolism
- Eye Abnormalities/genetics
- Eye Abnormalities/metabolism
- Eye Abnormalities/physiopathology
- Lithium Chloride/pharmacology
- Zebrafish/embryology*
- Zebrafish/metabolism
- Body Patterning/genetics
- Gene Expression Regulation, Developmental/drug effects
- Gene Expression Regulation, Developmental/genetics
- Gastrula/cytology
- Gastrula/metabolism
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism
- PubMed
- 17222974 Full text @ Neurosci. Lett.
Citation
Kim, H.S., Shin, J., Kim, S.H., Chun, H.S., Kim, J.D., Kim, Y.S., Kim, M.J., Rhee, M., Yeo, S.Y., and Huh, T.L. (2007) Eye field requires the function of Sfrp1 as a Wnt antagonist. Neuroscience letters. 414(1):26-29.
Abstract
Wnts have been shown to provide a posteriorizing signal that has to be repressed in the specification of vertebrate forebrain region. Previous studies have shown that Wnt activation by LiCl treatment causes an expansion of optic stalk and mid-hindbrain boundary, whereas eye and ventral diencephalon in the forebrain region were reduced. However, the molecular mechanism, by which inhibits Wnt activity in the forebrain remains poorly defined. To investigate relationship between forebrain specification and Wnt signaling, the zebrafish homologue of secreted frizzled related protein1 (sfrp1) has been characterized. The transcripts of sfrp1 are detected in the presumptive forebrain at gastrula and in the ventral telencephalon, ventral diencephalon, midbrain and optic vesicles at 24h after postfertilization (hpf). Overexpression of sfrp1 causes an anteriorization of embryo, with enlarged head and reduced posterior structure as in the embryo overexpressing dominant-negative form of Frizzled8a or Dkk1. Its overexpression restored the eye defects in the Wnt8b-overexpressing embryos, but not in the LiCl-treated embryos. These results suggest that Sfrp1 expressed in the forebrain and eye field plays a critical role in the extracellular events of antagonizing Wnt activity for the forebrain specification.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping