PUBLICATION

Expression of rasgef1b in zebrafish

Authors
Epting, D., Vorwerk, S., Hageman, A., and Meyer, D.
ID
ZDB-PUB-070210-23
Date
2007
Source
Gene expression patterns : GEP   7(4): 389-395 (Journal)
Registered Authors
Epting, Daniel, Hagemann, Anja, Meyer, Dirk, Vorwerk, Sabrina
Keywords
rasgef, rasgef1b, Zebrafish, Nodal, FGF, Germring, Somite, Rhombomere boundaries, Mid-hindbrain boundary, Inner nuclear layer
MeSH Terms
  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Brain/embryology
  • Brain/metabolism
  • Embryo, Nonmammalian/metabolism
  • Fibroblast Growth Factors/metabolism
  • Gene Expression Regulation, Developmental*
  • Humans
  • Molecular Sequence Data
  • Nodal Protein
  • Organ Specificity
  • Phylogeny
  • Protein Isoforms
  • Signal Transduction
  • Transcription Initiation Site
  • Transforming Growth Factor beta/metabolism
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish Proteins/genetics*
  • ras Guanine Nucleotide Exchange Factors/genetics*
(all 21)
PubMed
17239665 Full text @ Gene Expr. Patterns
Abstract
The rasgef genes encode a subgroup of highly conserved Ras guanine nucleotide exchange factors. While EST projects revealed the presence of rasgef genes in organisms that range from nematodes to humans, their functions remain to be elucidated. In zebrafish two rasgef genes, rasgef and rasgef1b, have been identified and high throughput analysis revealed tissue specific embryonic expression for rasgef1b. Here, we show that three rasgef1b-transcripts are generated from two transcriptional start sites and by alternative splicing. Detailed expression analyses show that rasgef1b is expressed in a subset of adaxial cells, in the anterior part of somites, in the rostral part of the mid-hindbrain boundary and in the rhombomere boundaries. In the larva, rasgef1b is further expressed in the pallium and the inner nuclear layer of the retina. We also find that rasgef1b is expressed maternally and that the ubiquitous distribution of maternal transcripts disappears shortly after mid-blastula transition. At early epiboly stages, rasgef1b expression is restricted to the margin with low levels of expression on the ventral and high levels of expression on the dorsal side. Finally, we show that early zygotic expression is regulated by Nodal and FGF signals and that these signals have different activities in regulating the level and distribution of early zygotic rasgef1b mRNA expression.
Genes / Markers
Figures
Figure Gallery (4 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
foxh1_unspecified
    Unspecified
    tdgf1_unspecified
      Unspecified
      1 - 2 of 2
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      Human Disease / Model
      Sequence Targeting Reagents
      No data available
      Fish
      Antibodies
      Orthology
      Engineered Foreign Genes
      No data available
      Mapping