PUBLICATION
How does blood cell concentration modulate cardiovascular parameters in developing zebrafish (Danio rerio)?
- Authors
- Kopp, R., Pelster, B., and Schwerte, T.
- ID
- ZDB-PUB-070122-11
- Date
- 2007
- Source
- Comparative biochemistry and physiology. Part A, Molecular & integrative physiology 146(3): 400-407 (Journal)
- Registered Authors
- Kopp, Renate, Pelster, Bernd, Schwerte, Thorsten
- Keywords
- Isovolemic anemia, Heart rate, Blood cell concentration, Vascular formation
- MeSH Terms
-
- Anemia/blood
- Animals
- Blood Cell Count*
- Blood Vessels/growth & development
- Blood Viscosity
- Erythropoiesis
- Heart/growth & development
- Zebrafish/blood*
- Zebrafish/growth & development*
- PubMed
- 17196857 Full text @ Comp. Biochem. Physiol. A Mol. Integr. Physiol.
Citation
Kopp, R., Pelster, B., and Schwerte, T. (2007) How does blood cell concentration modulate cardiovascular parameters in developing zebrafish (Danio rerio)?. Comparative biochemistry and physiology. Part A, Molecular & integrative physiology. 146(3):400-407.
Abstract
Blood flow and shear forces are considered to be important parameters possibly stimulating angiogenesis or cardiovascular remodeling. The main objective of this study was to test the hypothesis that a significant reduction in shear forces as a consequence of a significant isovolemic anemia induced by microsurgical techniques during early larval development of the zebrafish might induce a compensatory stimulation of erythropoiesis and/or induce a modification of cardiac activity or even the formation of the heart and may influence the shaping of the vascular bed. Blood from 2 day old zebrafish larvae was withdrawn and replaced by zebrafish Ringer's solution, so that the blood cell concentration was reduced by at least 75%. At 5 days post fertilization (dpf) a partial recovery in blood cell concentration was observed and reached a value of 814.55+/-85.42 cells/nL, while in control animals blood cell concentration amounted to 1856.00+/-131.59 cells/nL. At 7 dpf the value of blood cell concentration was 1023.89+/-95.75 cells/nL versus 1701.54+/-146.03 cells/nL in control animals. Compared to control animals, heart rate and cardiac output were significantly reduced in anemic animals and alterations in the formation of the vascular bed were also observed. A significant decrease in the end-diastolic volume suggested that ventricular volume was reduced. Thus, within a few days zebrafish larvae were nearly able to compensate for an isovolemic anemia by an enhanced erythropoiesis. However, several changes in cardiovascular system indicated that phenotypic plasticity is established even at an early developmental stage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping