PUBLICATION
Expression of Masu Salmon Delta5-Desaturase-Like Gene Elevated EPA and DHA Biosynthesis in Zebrafish
- Authors
- Alimuddin, Yoshizaki, G., Kiron, V., Satoh, S., and Takeuchi, T.
- ID
- ZDB-PUB-061227-14
- Date
- 2007
- Source
- Marine biotechnology (New York, N.Y.) 9(1): 92-100 (Journal)
- Registered Authors
- Keywords
- DHA, EPA, fatty acid Δ5 desaturase, masu salmon, transgenic, zebrafish
- MeSH Terms
-
- Actins/analysis
- Animal Feed/analysis
- Animals
- Animals, Genetically Modified/physiology*
- Artemia/chemistry
- Body Weight/physiology
- DNA Primers
- Docosahexaenoic Acids/analysis
- Docosahexaenoic Acids/metabolism
- Eicosapentaenoic Acid/analysis
- Eicosapentaenoic Acid/biosynthesis
- Fatty Acid Desaturases/biosynthesis*
- Fatty Acid Desaturases/genetics
- Fatty Acids, Omega-3/analysis
- Fatty Acids, Omega-3/biosynthesis*
- Female
- Food Technology*
- Gene Expression Regulation, Enzymologic/physiology*
- Lipids/analysis
- Male
- Oncorhynchus/genetics
- RNA, Messenger/analysis
- Zebrafish/physiology*
- PubMed
- 17136489 Full text @ Mar. Biotechnol.
Citation
Alimuddin, Yoshizaki, G., Kiron, V., Satoh, S., and Takeuchi, T. (2007) Expression of Masu Salmon Delta5-Desaturase-Like Gene Elevated EPA and DHA Biosynthesis in Zebrafish. Marine biotechnology (New York, N.Y.). 9(1):92-100.
Abstract
Farmed fish could substitute for marine capture fish as a source of fatty acids such as eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) beneficial for human health; however, they require these compounds in their diets. In the present study on a model fish species, we modified the EPA/DHA biosynthesis pathway by overexpression of masu salmon Delta5-desaturase-like gene in zebrafish to increase its ability to synthesize EPA and DHA. Expression of this gene in transgenic fish fed a commercial diet and Artemia helped to improve their EPA content by 1.21-fold and DHA by 1.24-fold. In similar fish that were fed only Artemia the increments were 1.14-fold for EPA and 1.13-fold for DHA, compared with nontransgenic fish. In contrast, eicosatetraenoic acid content decreased, as it is a substrate of Delta5-desaturase, while the total lipid remained constant. The results demonstrated that masu salmon Delta5-desaturase is functional in zebrafish and can modify its fatty acid metabolic pathway. The technique could be applied to farmed fish to generate a nutritionally richer product for human consumption.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping