PUBLICATION
Fluorescent protein expression driven by her4 regulatory elements reveals the spatiotemporal pattern of Notch signaling in the nervous system of zebrafish embryos
- Authors
- Yeo, S.Y., Kim, M., Kim, H.S., Huh, T.L., and Chitnis, A.B.
- ID
- ZDB-PUB-061227-11
- Date
- 2007
- Source
- Developmental Biology 301(2): 555-567 (Journal)
- Registered Authors
- Chitnis, Ajay, Huh, Tae-Lin, Kim, Hyung-Seok, Kim, Min Jung, Yeo, Sang-Yeob
- Keywords
- Notch reporter, Transgenic, Su(H), CBF1, CSL, her4, mind bomb, Neurogenin1, zath3, Neurogenesis
- MeSH Terms
-
- Cell Differentiation
- Zebrafish/embryology*
- Zebrafish/genetics
- Zebrafish/metabolism*
- Animals
- Luciferases/genetics
- Luciferases/metabolism
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Genes, Reporter/genetics
- Somites/metabolism
- Basic Helix-Loop-Helix Transcription Factors/genetics
- Basic Helix-Loop-Helix Transcription Factors/metabolism*
- Animals, Genetically Modified
- Receptors, Notch/genetics
- Receptors, Notch/metabolism*
- Signal Transduction*
- Gene Expression Regulation, Developmental
- Heterozygote
- Base Sequence
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Neurons/cytology
- Neurons/metabolism*
- Promoter Regions, Genetic/genetics
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- Ganglia/embryology
- Ganglia/metabolism
- Regulatory Elements, Transcriptional/genetics*
- PubMed
- 17134690 Full text @ Dev. Biol.
Citation
Yeo, S.Y., Kim, M., Kim, H.S., Huh, T.L., and Chitnis, A.B. (2007) Fluorescent protein expression driven by her4 regulatory elements reveals the spatiotemporal pattern of Notch signaling in the nervous system of zebrafish embryos. Developmental Biology. 301(2):555-567.
Abstract
Notch activation inhibits neuronal differentiation during development of the nervous system; however, the dynamic role of Notch signaling in individual cell lineages remains poorly understood. We have characterized 3.4 kb 5'-regulatory sequence of a Notch target gene, her4, and used it to drive fluorescent gene expression in transgenic lines where the spatiotemporal pattern of Notch activation can be examined in vivo. The 3.4 kb her4 promoter contains five predicted Su(H) binding sites of which two proximal sites were confirmed to be required for Notch-mediated transcriptional activation. Without Notch, Su(H) effectively represses transcription regulated by the promoter. Analyses of transgenic zebrafish showed that while the expression of proneural genes and Notch activation were both critical for endogenous her4 expression, reporter gene expression was primarily regulated by Notch activity. This study also showed that her4 may be differently regulated in sensory cranial ganglia, where Notch activity is not essential for her4 expression and where Su(H) may repress her4 expression. The establishment of a reporter line with Notch-Su(H)-dependent fluorescent gene expression provides a tool to explore the complex role of Notch signaling in the development of vertebrate nervous system.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping