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ZFIN ID: ZDB-PUB-061227-10
Targeted gene expression in the zebrafish prechordal plate
Inbal, A., Topczewski, J., and Solnica-Krezel, L.
Date: 2006
Source: Genesis (New York, N.Y. : 2000)   44(12): 584-588 (Journal)
Registered Authors: Inbal, Adi, Solnica-Krezel, Lilianna, Topczewski, Jacek
Keywords: Gal4-VP16, UAS, goosecoid, GFP
MeSH Terms:
  • Animals
  • Animals, Genetically Modified
  • Crosses, Genetic
  • Endoderm/metabolism*
  • Gene Expression*
  • In Situ Hybridization
  • Nervous System/embryology*
  • Nervous System/metabolism
  • Trans-Activators/genetics*
  • Zebrafish/embryology*
  • Zebrafish/metabolism
PubMed: 17133414 Full text @ Genesis
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ABSTRACT
Targeted gene expression is a powerful tool for understanding gene function in vivo. In zebrafish, overexpression of gene products is typically accomplished ubiquitously, without temporal and spatial specificity. However, the yeast Gal4/UAS system can be used for targeted gene expression in zebrafish. Here we describe the generation and characterization of Tg[gsc: Gal4-VP16] transgenic zebrafish lines that harbor a construct encoding Gal4-VP16 transcriptional activator under the control of a fragment of the goosecoid gene promoter. Tg[gsc:Gal4-VP16] embryos express Gal4-VP16 RNA in presumptive prechordal plate mesendoderm during late blastula and throughout gastrulation. By crossing these fish to Tg[UAS-GFP] transgenic fish, we show that the gsc:Gal4-VP16 transgene is capable of driving strong expression of a target gene in the prechordal plate and its derivatives during gastrulation and segmentation. Thus, the use of Tg[gsc:Gal4-VP16] fish can help in understanding gene function in the prechordal plate, an embryonic structure that is crucial for normal neural patterning.
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