PUBLICATION
The in vivo form of the murine class VI POU protein Emb is larger than that encoded by previously described transcripts
- Authors
- Relaix, F., Molinari, S., Lemonnier, M., Schafer, B., and Buckingham, M.
- ID
- ZDB-PUB-061222-6
- Date
- 2004
- Source
- Gene 333: 35-46 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Alternative Splicing
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Western
- Cell Line
- Cell Line, Tumor
- Cloning, Molecular
- DNA, Complementary/chemistry
- DNA, Complementary/genetics
- DNA-Binding Proteins/genetics*
- DNA-Binding Proteins/metabolism
- Genes/genetics
- Humans
- Molecular Sequence Data
- POU Domain Factors
- Protein Isoforms/genetics
- Rats
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Transcription Factors/genetics*
- Transcription Factors/metabolism
- Transcription, Genetic/genetics
- PubMed
- 15177678 Full text @ Gene
Citation
Relaix, F., Molinari, S., Lemonnier, M., Schafer, B., and Buckingham, M. (2004) The in vivo form of the murine class VI POU protein Emb is larger than that encoded by previously described transcripts. Gene. 333:35-46.
Abstract
The class VI POU domain family member known as Emb in the mouse (rat Brn5 or human mPOU/TCFbeta1) is present in vivo as a protein migrating at about 80 kDa on western blots, considerably larger than that predicted (about 42 kDa) from previously cloned coding sequences. By RT-PCR and 5' RACE strategies a full-length Emb sequence, Emb FL, is now identified. Shorter sequences encoding the -COOH terminal, and an -NH(2) terminal isoform, EmbN, were also isolated. Comparisons of Emb coding sequences between species, including the full-length zebra fish, POU(c), are presented, together with a compilation of the multiple transcripts produced by alternative splicing and the presence of different transcriptional start and stop sites, from the Emb gene.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping