header logo image header logo text
Downloads Login
General Information
ZFIN ID: ZDB-PUB-061205-4
The zebrafish genome contains two inducible, functional cyclooxygenase-2 genes
Ishikawa, T.O., Griffin, K.J., Banerjee, U., and Herschman, H.R.
Date: 2007
Source: Biochemical and Biophysical Research Communications 352(1): 181-187 (Journal)
Registered Authors: Griffin, Kevin
Keywords: none
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • COS Cells
  • Cell Line
  • Cercopithecus aethiops
  • Conserved Sequence
  • Cyclooxygenase 2/chemistry
  • Cyclooxygenase 2/genetics*
  • Cyclooxygenase 2/metabolism*
  • Genome/genetics*
  • Isoenzymes/chemistry
  • Isoenzymes/genetics
  • Isoenzymes/metabolism
  • Molecular Sequence Data
  • Phylogeny
  • Sequence Alignment
  • Zebrafish/genetics*
PubMed: 17112475 Full text @ Biochem. Biophys. Res. Commun.
Cyclooxygenase is a key enzyme in prostanoid biosynthesis. Mammalian species have two cyclooxygenases, constitutively expressed cyclooxygenase-1 (Cox-1) and inducible cyclooxygenase-2 (Cox-2). Cox-1 and/or Cox-2 have been also identified in other vertebrates, including fish. We identified a second zebrafish Cox-2 gene orthologue, Cox-2b. All of the functionally important amino acids for cyclooxygenase enzymes are conserved in Cox-2b. The 3' untranslated region of the Cox-2b message contains AU rich elements characteristic of regulation at the level of mRNA stability. Constitutive tissue expression patterns for Cox-2a and Cox-2b are distinct, but overlap. Both Cox-2a and Cox-2b expression are inducible in the kidney when fish are exposed to tetradecanoylphorbol acetate. Like Cox-2a, Cox-2b protein, expressed in COS cells is functionally active. Thus, the zebrafish genome contains two functional, inducible Cox-2 genes. Database searching demonstrates that some fish genomes contain multiple Cox-1 or Cox-2 cyclooxygenase genes, suggesting alternate duplication and retention of this gene.