PUBLICATION
Profiling of prostanoids in zebrafish embryonic development
- Authors
- Yeh, H.C., and Wang, L.H.
- ID
- ZDB-PUB-060927-12
- Date
- 2006
- Source
- Prostaglandins, leukotrienes, and essential fatty acids 75(6): 397-402 (Journal)
- Registered Authors
- Keywords
- none
- MeSH Terms
-
- Animals
- Embryo, Nonmammalian/metabolism
- Embryonic Development*/genetics
- Prostaglandin-Endoperoxide Synthases/genetics
- Prostaglandin-Endoperoxide Synthases/metabolism
- Prostaglandins/analysis
- Prostaglandins/metabolism*
- RNA, Messenger/analysis
- RNA, Messenger/metabolism
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/embryology*
- Zebrafish/metabolism
- PubMed
- 17000094 Full text @ Prostaglandins Leukot. Essent. Fatty Acids
Citation
Yeh, H.C., and Wang, L.H. (2006) Profiling of prostanoids in zebrafish embryonic development. Prostaglandins, leukotrienes, and essential fatty acids. 75(6):397-402.
Abstract
Prostanoids (PG) play important roles in vascular, pulmonary, reproductive and renal physiology. Little is known about their roles in the embryonic development. Using the oviparous zebrafish embryo as a model, we determined the temporal expression of PGs synthesized from exogenous prostaglandin H(2). Prostaglandin E(2) is the major PG throughout first 120h post-fertilization (hpf), whereas prostaglandin F(2)(alpha) is at a lower but also a constant level. Reverse transcription-polymerase chain reaction (RT-PCR) showed that transcripts of cytosolic and membrane-bound PGE synthases were evident during the 120hpf period. Compared with thromboxane A(2), the level of prostacyclin (PGI(2))is higher at first 24hpf, the stage before the formation of blood vessel. RT-PCR showed that transcript of prostacyclin synthase appeared at 7hpf whereas thromboxane synthase appeared at 48hpf, suggesting that PGI(2) has additional functions besides hemostasis. Interestingly, level of prostaglandin D(2) (PGD(2)) followed an exponential decay over 120hpf with a rate constant of 0.048h(-1) and transcript of lipocalin-type PGD synthase was expressed at a higher level at early stage of development, suggesting that PGD(2) is highly regulated during embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping