PUBLICATION

Sp5l is a mediator of Fgf signals in anteroposterior patterning of the neuroectoderm in zebrafish embryo

Authors
Sun, Z., Zhao, J., Zhang, Y., and Meng, A.
ID
ZDB-PUB-060911-5
Date
2006
Source
Developmental Dynamics : an official publication of the American Association of Anatomists   235(11): 2999-3006 (Journal)
Registered Authors
Meng, Anming, Sun, Zhihui, Zhang, Yu, Zhao, Jue
Keywords
zebrafish, fibroblast growth factor, Sp1-like transcription factor, neuroectoderm, patterning
MeSH Terms
  • Sp Transcription Factors/analysis
  • Sp Transcription Factors/genetics
  • Sp Transcription Factors/physiology*
  • Zebrafish/embryology*
  • Zebrafish/genetics
  • Zebrafish/metabolism
  • Gene Expression Regulation, Developmental*
  • Body Patterning/genetics*
  • Animals
  • Fibroblast Growth Factors/antagonists & inhibitors
  • Fibroblast Growth Factors/genetics*
  • Nervous System/chemistry
  • Nervous System/embryology*
  • Nervous System/metabolism
  • Embryo, Nonmammalian/metabolism
  • Embryonic Development/genetics
  • Ectoderm/chemistry
  • Ectoderm/metabolism
  • Signal Transduction
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism
  • Zebrafish Proteins/analysis
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/physiology*
(all 24)
PubMed
16958103 Full text @ Dev. Dyn.
Abstract
The neuroectoderm is patterned along the anterior-posterior axis in vertebrate embryos. Fgf signals are required to induce the posterior neuroectodermal fates, but they repress the anterior fate. Sp5l/Spr2, an Sp1-like transcription factor family member, has been shown to be required for development of mesoderm and posterior neuroectoderm. We demonstrate here that repression of the anterior neuroectodermal markers fez and otx1 by fgf17b or fgf3 coincides with induction of sp5l in the anterior neuroectoderm, and that this repression is efficiently rescued by simultaneous sp5l knockdown. On the other hand, sp5l knockdown is able to inhibit inductive activity of ectopic Fgf signals on the expression of the posterior neuroectodermal markers gbx2, hoxb1b, and krox20. Furthermore, effect of overexpression of a dominant negative Fgf receptor on anteroposterior patterning of the neuroectoderm is rescued by sp5l overexpression. Taken together, these data suggest that sp5l mediates the functions of Fgf signals in anteroposterior patterning of the neuroectoderm during zebrafish embryogenesis.
Genes / Markers
Figures
Figure Gallery (6 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
No data available
Human Disease / Model
No data available
Sequence Targeting Reagents
Target Reagent Reagent Type
sp5lMO2-sp5lMRPHLNO
1 - 1 of 1
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Fish
Fish
TU
TU + MO2-sp5l
1 - 2 of 2
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Antibodies
No data available
Orthology
No data available
Engineered Foreign Genes
No data available
Mapping
No data available
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Citation
Sun, Z., Zhao, J., Zhang, Y., and Meng, A. (2006) Sp5l is a mediator of Fgf signals in anteroposterior patterning of the neuroectoderm in zebrafish embryo. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(11):2999-3006.