PUBLICATION

A conserved NXIP motif is required for cell adhesion properties of the syndecan-4 ectodomain

Authors
Whiteford, J.R., and Couchman, J.R.
ID
ZDB-PUB-060906-8
Date
2006
Source
The Journal of biological chemistry   281(43): 32156-32163 (Journal)
Registered Authors
Keywords
none
MeSH Terms
  • Conserved Sequence
  • Amino Acid Motifs
  • Dogs
  • Skin/cytology
  • Chlorocebus aethiops
  • Molecular Sequence Data
  • COS Cells
  • Mice
  • Zebrafish/genetics
  • Cell Line, Tumor
  • Jurkat Cells
  • Female
  • Cell Culture Techniques
  • Cell Adhesion*
  • Cricetinae
  • Glutathione Transferase/metabolism
  • K562 Cells
  • Cell Line
  • Sequence Analysis, Protein
  • Animals
  • Amino Acid Sequence
  • Endothelial Cells/cytology
  • Endothelial Cells/physiology
  • Cloning, Molecular
  • Protein Structure, Tertiary
  • Humans
  • Syndecan-4/chemistry*
  • Syndecan-4/genetics*
  • Syndecan-4/isolation & purification
  • Syndecan-4/metabolism
  • Syndecan-4/physiology*
  • Sequence Homology, Amino Acid
  • Fibroblasts/cytology
  • Fibroblasts/physiology
  • 3T3 Cells
  • Rats
  • Recombinant Fusion Proteins/isolation & purification
  • Recombinant Fusion Proteins/metabolism
  • Breast Neoplasms/pathology
  • Plasmids
(all 40)
PubMed
16936286 Full text @ J. Biol. Chem.
Abstract
Syndecans are cell surface proteoglycans involved in cell adhesion and motility. Syndecan-4 is an important component of focal adhesions and is involved in cytoskeletal reorganisation. Previous work has shown that the syndecan-4 ectodomain can support cell attachment. Here, three vertebrate syndecan-4 ectodomains are compared including that of the zebrafish and we demonstrate that the cell binding activity of the syndecan-4 ectodomain is conserved. Cell adhesion to the syndecan-4 ectodomain appears to be a characteristic of mesenchymal cells. Comparison of syndecan-4 ectodomain sequences lead to the identification of three conserved regions of sequence of which one, an NXIP motif, is important for cell binding activity. We show that cell adhesion to the syndecan-4 ectodomain involves ss1 integrins in several cell types.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Whiteford, J.R., and Couchman, J.R. (2006) A conserved NXIP motif is required for cell adhesion properties of the syndecan-4 ectodomain. The Journal of biological chemistry. 281(43):32156-32163.