PUBLICATION
Small molecule-induced ablation and subsequent regeneration of larval zebrafish melanocytes
- Authors
- Yang, C.T., and Johnson, S.L.
- ID
- ZDB-PUB-060825-7
- Date
- 2006
- Source
- Development (Cambridge, England) 133(18): 3563-3573 (Journal)
- Registered Authors
- Johnson, Stephen L., Yang, Chao-Tsung
- Keywords
- Melanocyte, Regeneration, Chemical ablation, Stem cell, Cell division
- MeSH Terms
-
- In Situ Hybridization/methods
- Zebrafish/genetics*
- Zebrafish/metabolism
- Melanocytes/cytology
- Melanocytes/drug effects*
- Melanocytes/metabolism
- Morpholines/chemistry
- Morpholines/pharmacology*
- Mutation/genetics
- Larva/cytology
- Larva/drug effects
- Larva/metabolism
- Stem Cells/cytology
- Stem Cells/drug effects
- Stem Cells/metabolism
- Gene Expression Regulation, Developmental/drug effects
- Cells, Cultured
- Monophenol Monooxygenase/metabolism
- Phenols/chemistry
- Phenols/pharmacology*
- Phenols/toxicity*
- Cell Division/drug effects
- Cell Division/genetics
- Cell Survival/drug effects
- Cell Survival/genetics
- Animals
- Cell Differentiation/drug effects
- Cell Differentiation/genetics
- Sulfhydryl Compounds/chemistry
- Sulfhydryl Compounds/toxicity*
- PubMed
- 16914496 Full text @ Development
Citation
Yang, C.T., and Johnson, S.L. (2006) Small molecule-induced ablation and subsequent regeneration of larval zebrafish melanocytes. Development (Cambridge, England). 133(18):3563-3573.
Abstract
We developed a method to efficiently ablate a single cell type, the zebrafish melanocyte, and study the mechanisms of its regeneration. We found that a small molecule, (2-morpholinobutyl)-4-thiophenol (MoTP), specifically ablates zebrafish larval melanocytes or melanoblasts, and that this melanocytotoxicity is dependent on tyrosinase activity, which presumably converts MoTP to cytotoxic quinone species. Following melanocyte ablation by MoTP treatment, we demonstrate by BrdU incorporation experiments that regenerated melanocytes are derived from the division of otherwise quiescent melanocyte precursors or stem cells. We further show that larval melanocyte regeneration requires the kit receptor tyrosine kinase. Our results suggest that a small number of melanocyte precursors or stem cells unevenly distributed in larvae are drawn upon to reconstitute the larval melanocyte population following melanocyte ablation by MoTP.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping