Zebrafish promoter microarrays identify actively transcribed embryonic genes
- Wardle, F.C., Odom, D.T., Bell, G.W., Yuan, B., Danford, T.W., Wiellette, E.L., Herbolsheimer, E., Sive, H.L., Young, R.A., and Smith, J.C.
- Genome biology 7(8): R71 (Journal)
- Registered Authors
- Sive, Hazel, Smith, Jim, Wardle, Fiona, Wiellette, Elizabeth
- MeSH Terms
- Chromatin Immunoprecipitation
- Embryo, Nonmammalian/embryology
- Embryo, Nonmammalian/metabolism
- Gene Expression Regulation, Developmental*
- Oligonucleotide Array Sequence Analysis
- Promoter Regions, Genetic/genetics*
- Reverse Transcriptase Polymerase Chain Reaction
- 16889661 Full text @ Genome Biol.
Wardle, F.C., Odom, D.T., Bell, G.W., Yuan, B., Danford, T.W., Wiellette, E.L., Herbolsheimer, E., Sive, H.L., Young, R.A., and Smith, J.C. (2006) Zebrafish promoter microarrays identify actively transcribed embryonic genes. Genome biology. 7(8):R71.
ABSTRACT: We have designed a zebrafish genomic microarray to identify DNA-protein interactions in the proximal promoter regions of over 11,000 zebrafish genes. Using these microarrays, together with chromatin immunoprecipitation with an antibody directed against tri-methylated lysine 4 of Histone H3, we demonstrate the feasibility of this method in zebrafish. This approach will allow investigators to determine the genomic binding locations of DNA interacting proteins during development and expedite the assembly of the genetic networks that regulate embryogenesis.
Errata / NotesLab website for supplementary data.
Genes / Markers
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Engineered Foreign Genes