PUBLICATION
Embryonic expression of zebrafish AMPA receptor genes: Zygotic gria2alpha expression initiates at the midblastula transition
- Authors
- Lin, W.H., Wu, C.H., Chen, Y.C., and Chow, W.Y.
- ID
- ZDB-PUB-060807-11
- Date
- 2006
- Source
- Brain research 1110(1): 46-54 (Journal)
- Registered Authors
- Keywords
- AMPA receptor, Gene expression, RNA editing, C-terminal splice form, Midblastula transition, Zebrafish embryogenesis
- MeSH Terms
-
- Embryo, Nonmammalian
- RNA Editing/physiology
- Animals
- Sequence Analysis, Protein
- Blastula/physiology*
- Zebrafish
- RNA, Messenger/metabolism
- Gene Expression Regulation, Developmental/physiology*
- Reverse Transcriptase Polymerase Chain Reaction/methods
- Gene Expression/physiology*
- Receptors, AMPA/genetics
- Receptors, AMPA/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 16887104 Full text @ Brain Res.
Citation
Lin, W.H., Wu, C.H., Chen, Y.C., and Chow, W.Y. (2006) Embryonic expression of zebrafish AMPA receptor genes: Zygotic gria2alpha expression initiates at the midblastula transition. Brain research. 1110(1):46-54.
Abstract
The AMPA-preferring receptors (AMPARs) mediate rapid excitatory synaptic transmission in the central nervous system of vertebrates. Expression profiles of 8 AMPAR genes were studied by RT-PCR analyses to elucidate the properties of AMPARs during early zebrafish development. Transcripts of all AMPAR genes are detected at the time of fertilization, suggesting maternal transcriptions of zebrafish AMPAR genes. The amounts of gria1 and gria2 transcripts are several-fold higher than that of gria3 and gria4 between 10 and 72 hpf (hour postfertilization). The edited gria2alpha transcript decreases during gastrulation period, suggesting that zygotic expression of gria2alpha begins around the time of midblastula transition. Relative to the amount of beta-actin, the amounts of AMPAR transcripts increase significantly after the completion of neurulation. The amounts of gria2 transcripts exceed the total amounts of the remaining AMPAR transcripts after 36 hpf, suggesting increases in the representation of low Ca(2+) permeable AMPARs during neuronal maturation. Many but not all of the known mammalian protein-protein interaction motifs are preserved in the C-terminal domains (CTD) of zebrafish AMPARs. Before 16 hpf, the embryos express predominantly the alternative splice forms encoding longer CTD. Representations of the short CTD splice forms of gria2 and gria4alpha increase after 24 hpf, when neurulation is nearly completed.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping