PUBLICATION

FGF signaling is required for {beta}-catenin-mediated induction of the zebrafish organizer

Authors
Maegawa, S., Varga, M., and Weinberg, E.S.
ID
ZDB-PUB-060731-20
Date
2006
Source
Development (Cambridge, England)   133(16): 3265-3276 (Journal)
Registered Authors
Maegawa, Shingo, Weinberg, Eric
Keywords
β-Catenin, FGF signaling, Dorsal axis formation, Neural induction, Dorsoventral patterning, Anteroposterior patterning, Organizer, Nodal, Chordin, Zebrafish
MeSH Terms
  • Intercellular Signaling Peptides and Proteins/metabolism
  • Bone Morphogenetic Protein 4
  • Embryo, Nonmammalian/metabolism
  • Animals
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/metabolism
  • Bone Morphogenetic Proteins/metabolism
  • beta Catenin/agonists
  • beta Catenin/metabolism*
  • Transcription, Genetic
  • Glycoproteins/metabolism
  • Signal Transduction
  • Body Patterning
  • Zebrafish/embryology*
  • Homeodomain Proteins/genetics
  • Homeodomain Proteins/metabolism
  • Fibroblast Growth Factor 3/antagonists & inhibitors
  • Fibroblast Growth Factor 3/genetics
  • Fibroblast Growth Factor 3/metabolism*
  • Nodal Signaling Ligands
  • Organizers, Embryonic/metabolism*
  • Fibroblast Growth Factor 8/antagonists & inhibitors
  • Fibroblast Growth Factor 8/genetics
  • Fibroblast Growth Factor 8/metabolism*
(all 24)
PubMed
16873584 Full text @ Development
Abstract
We have used the maternal effect mutant ichabod, which is deficient in maternal beta-catenin signaling, to test for the epistatic relationship between beta-catenin activation, FGF signaling and bozozok, squint and chordin expression. Injection of beta-catenin RNA into ichabod embryos can completely rescue normal development. By contrast, when FGF signaling is inhibited, beta-catenin did not induce goosecoid and chordin, repress bmp4 expression or induce a dorsal axis. These results demonstrate that FGF signaling is necessary for beta-catenin induction of the zebrafish organizer. We show that FGFs function downstream of squint and bozozok to turn on chordin expression. Full rescue of ichabod by Squint is dependent on FGF signaling, and partial rescue by FGFs is completely dependent on chordin. By contrast, Bozozok can rescue the complete anteroposterior axis, but not notochord, in embryos blocked in FGF signaling. Surprisingly, accumulation of bozozok transcript in beta-catenin RNA-injected ichabod embryos is also dependent on FGF signaling, indicating a role of FGFs in maintenance of bozozok RNA. These experiments show that FGF-dependent organizer function operates through both bozozok RNA accumulation and a pathway consisting of beta-catenin-->Squint-->FGF-->Chordin, in which each component is sufficient for expression of the downstream factors of the pathway, and in which Nodal signaling is required for FGF gene expression and FGF signaling is required for Squint induction of chordin.
Genes / Markers
Figures
Figure Gallery (8 images)
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Expression
Phenotype
No data available
Mutations / Transgenics
Allele Construct Type Affected Genomic Region
b2
    		Unknown
    p1
      		Unknown
      1 - 2 of 2
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      Human Disease / Model
      No data available
      Sequence Targeting Reagents
      Target Reagent Reagent Type
      chrdMO1-chrdMRPHLNO
      ndr1MO4-ndr1MRPHLNO
      ndr2MO1-ndr2MRPHLNO
      1 - 3 of 3
      Show
      Fish
      Fish
      AB
      brsb2 (AB)
      ctnnb2p1
      WT
      1 - 4 of 4
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      Antibodies
      No data available
      Orthology
      No data available
      Engineered Foreign Genes
      No data available
      Mapping
      No data available
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      Citation
      Maegawa, S., Varga, M., and Weinberg, E.S. (2006) FGF signaling is required for {beta}-catenin-mediated induction of the zebrafish organizer. Development (Cambridge, England). 133(16):3265-3276.