PUBLICATION
In vitro male germ cell cultures of zebrafish
- Authors
- Sakai, N.
- ID
- ZDB-PUB-060719-10
- Date
- 2006
- Source
- Methods (San Diego, Calif.) 39(3): 239-245 (Review)
- Registered Authors
- Sakai, Noriyoshi
- Keywords
- none
- MeSH Terms
-
- Animals
- Animals, Genetically Modified/genetics
- Cell Differentiation
- Coculture Techniques*
- Fertilization in Vitro
- Genetic Engineering/methods*
- Male
- Retroviridae/genetics
- Sertoli Cells/cytology
- Spermatozoa/cytology*
- Spermatozoa/enzymology
- Zebrafish*
- beta-Galactosidase/metabolism
- PubMed
- 16828310 Full text @ Methods
Citation
Sakai, N. (2006) In vitro male germ cell cultures of zebrafish. Methods (San Diego, Calif.). 39(3):239-245.
Abstract
Transgenic modification of sperm before fertilization has the advantages of a much shorter timeline for the production of transgenic animals. A culture system using primary cultures of zebrafish male germ cells, in which the differentiation of spermatogonia to functional sperm can occur in vitro, allows us to introduce foreign DNA into the cultured sperm and to produce transgenics from the sperm. This chapter describes methods for the co-culture of male germ cells and a Sertoli cell feeder layer and the introduction of foreign DNA with retroviruses. This male germ cell culture system should prove useful not only in producing genetically modified sperm, but also in analyzing the regulatory function of Sertoli cells for spermatogenesis in vertebrates.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping