PUBLICATION
Molecular Cloning of a Novel Gene ZAhi-1 and its Expression Analysis during Zebrafish Gametogenesis
- Authors
- Zhou, W., and Song, P.
- ID
- ZDB-PUB-060710-8
- Date
- 2006
- Source
- Molecular biology reports 33(2): 111-116 (Journal)
- Registered Authors
- Song, Ping, Zhou, Wei
- Keywords
- Ahi-1, cDNA cloning, in situ hybridization, RT-PCR, zebrafish
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Carrier Proteins/genetics*
- Carrier Proteins/metabolism*
- Cloning, Molecular
- Expressed Sequence Tags
- Gametogenesis*
- Gene Expression Regulation, Developmental
- In Situ Hybridization
- Mice
- Molecular Sequence Data
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Homology, Amino Acid
- Tissue Distribution
- Zebrafish/embryology*
- Zebrafish Proteins/genetics*
- Zebrafish Proteins/metabolism*
- PubMed
- 16817020 Full text @ Mol. Biol. Rep.
Citation
Zhou, W., and Song, P. (2006) Molecular Cloning of a Novel Gene ZAhi-1 and its Expression Analysis during Zebrafish Gametogenesis. Molecular biology reports. 33(2):111-116.
Abstract
Proto-oncogen Ahi-1 is closely related to a lot of human and mouse diseases. Ahi-1 mutation will lead to leukemia in mice and Joubert syndrome in human beings. We have cloned the full cDNA sequence of Ahi-1 homologous in zebrafish, and RT-PCR results of ZAhi-1 in different tissues reveal that ZAhi-1 expressed highest in the mature gonad. In situ hybridization results of zebrafish gonad show that ZAhi-1 only expressed in the early stages' gamete cells. RT-PCR analyses of mouse Ahi-1 in different stages of spermatogenesis have been done according to the published Ahi-1 sequence, and the findings reveal that Ahi-1 is expressed in gamete of pachytene stage. It can then be safely concluded that Ahi-1 might take place in the spermatocyte from the early pachytene stage to the late pachytene stage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping