PUBLICATION
Origin of the tetraspanin uroplakins and their co-evolution with associated proteins: Implications for uroplakin structure and function
- Authors
- Garcia-Espana, A., Chung, P.J., Zhao, X., Lee, A., Pellicer, A., Yu, J., Sun, T.T., and Desalle, R.
- ID
- ZDB-PUB-060710-3
- Date
- 2006
- Source
- Molecular phylogenetics and evolution 41(2): 355-367 (Journal)
- Registered Authors
- Keywords
- Genomics, Uroplakin, Tetrapods, Mutigene family, Phylogenetics, Protein evolution
- MeSH Terms
-
- Chickens/genetics
- Animals
- Phylogeny
- Evolution, Molecular*
- Gene Duplication
- Sequence Alignment
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology
- Uroplakin III
- Molecular Sequence Data
- Gene Expression Regulation
- Humans
- Membrane Glycoproteins/chemistry
- Membrane Glycoproteins/genetics*
- Membrane Glycoproteins/physiology*
- Membrane Proteins/genetics
- Membrane Proteins/physiology
- Amino Acid Sequence
- Urothelium/chemistry
- Xenopus Proteins/chemistry
- Xenopus Proteins/genetics
- Xenopus Proteins/physiology
- Avian Proteins/chemistry
- Avian Proteins/genetics
- Avian Proteins/physiology
- Uroplakin II
- Zebrafish/genetics
- Xenopus laevis/genetics
- PubMed
- 16814572 Full text @ Mol. Phylogenet. Evol.
Citation
Garcia-Espana, A., Chung, P.J., Zhao, X., Lee, A., Pellicer, A., Yu, J., Sun, T.T., and Desalle, R. (2006) Origin of the tetraspanin uroplakins and their co-evolution with associated proteins: Implications for uroplakin structure and function. Molecular phylogenetics and evolution. 41(2):355-367.
Abstract
Genome level information coupled with phylogenetic analysis of specific genes and gene families allow for a better understanding of the structure and function of their protein products. In this study, we examine the mammalian uroplakins (UPs) Ia and Ib, members of the tetraspanin superfamily, that interact with uroplakins UPII and UPIIIa/IIIb, respectively, using a phylogenetic approach of these genes from whole genome sequences. These proteins interact to form urothelial plaques that play a central role in the permeability barrier function of the apical urothelial surface of the urinary bladder. Since these plaques are found exclusively in mammalian urothelium, it is enigmatic that UP-like genomic sequences were recently found in lower vertebrates without a typical urothelium. We have cloned full-length UP-related cDNAs from frog (Xenopus laevis), chicken (Gallus gallus), and zebrafish (Danio rerio), and combined these data with sequence information from their orthologs in all the available fully sequenced and annotated animal genomes. Phylogenetic analyses of all the available uroplakin sequences, and an understanding of their distribution in several animal taxa, suggest that: (i) the UPIa/UPIb and UPII/UPIII genes evolved by gene duplication in the common ancestor of vertebrates; (ii) uroplakins can be lost in different combinations in vertebrate lineages; and (iii) there is a strong co-evolutionary relationship between UPIa and UPIb and their partners UPII and UPIIIa/IIIb, respectively. The co-evolution of the tetraspanin UPs and their associated proteins may fine-tune the structure and function of uroplakin complexes enabling them to perform diverse species- and tissue-specific functions. The structure and function of uroplakins, which are also expressed in Xenopus kidney, oocytes and fat body, are much more versatile than hitherto appreciated.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping