PUBLICATION
Methanol alters ecto-nucleotidases and acetylcholinesterase in zebrafish brain
- Authors
- Rico, E.P., Rosemberg, D.B., Senger, M.R., de Bem Arizi, M., Bernardi, G.F., Dias, R.D., Bogo, M.R., and Bonan, C.D.
- ID
- ZDB-PUB-060703-11
- Date
- 2006
- Source
- Neurotoxicology and teratology 28(4): 489-496 (Journal)
- Registered Authors
- Keywords
- Methanol, Ecto-nucleotidase, Acetylcholinesterase, NTPDase, Ecto-52-nucleotidase, Zebrafish
- MeSH Terms
-
- 5'-Nucleotidase/biosynthesis
- 5'-Nucleotidase/metabolism*
- Acetylcholinesterase/biosynthesis
- Acetylcholinesterase/metabolism*
- Adenosine Triphosphate/metabolism
- Animals
- Brain/drug effects
- Brain/enzymology*
- Enzyme Induction
- Female
- Male
- Methanol/pharmacology*
- Nerve Tissue Proteins/biosynthesis
- Phylogeny
- Reverse Transcriptase Polymerase Chain Reaction
- Zebrafish/physiology*
- PubMed
- 16806813 Full text @ Neurotoxicol. Teratol.
Citation
Rico, E.P., Rosemberg, D.B., Senger, M.R., de Bem Arizi, M., Bernardi, G.F., Dias, R.D., Bogo, M.R., and Bonan, C.D. (2006) Methanol alters ecto-nucleotidases and acetylcholinesterase in zebrafish brain. Neurotoxicology and teratology. 28(4):489-496.
Abstract
Methanol is a neurotoxic compound that is responsible for serious damage on CNS. Besides being found as an environmental contaminant, this alcohol is also employed as a component of cryoprotector solutions for zebrafish embryos. Here we tested the acute effect of methanol on ecto-nucleotidase (NTPDase, ecto-5'-nucleotidase) and acetylcholinesterase (AChE) activities in zebrafish brain. After acute treatment, there were significant decreases on ATP (26% and 45%) and ADP hydrolysis (26% and 30%) at 0.5% and 1.0%, respectively. However, no significant alteration on ecto-5'-nucleotidase activity was verified in zebrafish brain. A significant inhibition on AChE activity (39%, 33% and 30%) was observed at the range of 0.25% to 1.0% methanol exposure. Four NTPDase sequences were identified from phylogenetic analyses, which one is similar to NTPDase1 and the others to NTPDase2. Methanol was able to inhibit NTPDase1, two isoforms of NTPDase2 and AChE transcripts. To evaluate if methanol affects directly these enzymes activities, we have performed in vitro assays. ATP hydrolysis presented a significant inhibition (19% and 34%) at 1.5% and 3.0%, respectively, and ADP hydrolysis decreased only at 3.0% (29.2%). Nevertheless, AMP hydrolysis and AChE were not altered after in vitro exposure. The inhibitory effect observed on these enzymes could contribute to the neurodegenerative events promoted by methanol in zebrafish brain.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping