PUBLICATION

Cysteine and tyrosine-rich 1 (CYYR1), a novel unpredicted gene on human chromosome 21 (21q21.2), encodes a cysteine and tyrosine-rich protein and defines a new family of highly conserved vertebrate-specific genes

Authors
Vitale, L., Casadei, R., Canaider, S., Lenzi, L., Strippoli, P., D'Addabbo, P., Giannone, S., Carinci, P., and Zannotti, M.
ID
ZDB-PUB-060615-3
Date
2002
Source
Gene   290(1-2): 141-151 (Journal)
Registered Authors
Casadei, Raffaella
Keywords
none
MeSH Terms
  • Phylogeny
  • Blotting, Northern
  • Amino Acid Sequence
  • Vertebrates/genetics*
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Evolution, Molecular
  • Expressed Sequence Tags
  • Molecular Sequence Data
  • Proteins/genetics*
  • Gene Expression
  • Sequence Analysis, DNA
  • Chromosomes, Human, Pair 21/genetics*
  • DNA, Complementary/chemistry
  • DNA, Complementary/genetics
  • Animals
  • RNA, Messenger/genetics
  • RNA, Messenger/metabolism
  • Radiation Hybrid Mapping
  • Female
  • Humans
  • Membrane Proteins
  • Databases, Nucleic Acid
  • Mice
(all 24)
PubMed
12062809 Full text @ Gene
Abstract
A novel human gene has been identified by in-depth bioinformatics analysis of chromosome 21 segment 40/105 (21q21.1), with no coding region predicted in any previous analysis. Brain-derived DNA complementary to RNA (cDNA) sequencing predicts a 154-amino acid product with no similarity to any known protein. The gene has been named cysteine and tyrosine-rich protein 1 gene (symbol cysteine and tyrosine-rich 1, CYYR1). The CYYR1 messenger RNA was found by Northern blot analysis in a broad range of tissues (two transcripts of 3.4 and 2.2 kb). The gene consists of four exons and spans about 107 kb, including a very large intron of 85.8 kb. Analysis of expressed sequence tags shows high CYYR1 expression in cells belonging to the amine precursor uptake and decarboxylation system. We also cloned the cDNA of the murine ortholog Cyyr1, which was mapped by a radiation hybrid panel on chromosome 16 within the region corresponding to that containing the respective human homolog on chromosome 21. Sequence and phylogenetic analysis led to identification of several genes encoding CYYR1 homologous proteins. The most prominent feature identified in the protein family is a central, unique cysteine and tyrosine-rich domain, which is strongly conserved from lower vertebrates (fishes) to humans but is absent in bacteria and invertebrates.
Genes / Markers
Figures
No images available
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
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Citation
Vitale, L., Casadei, R., Canaider, S., Lenzi, L., Strippoli, P., D'Addabbo, P., Giannone, S., Carinci, P., and Zannotti, M. (2002) Cysteine and tyrosine-rich 1 (CYYR1), a novel unpredicted gene on human chromosome 21 (21q21.2), encodes a cysteine and tyrosine-rich protein and defines a new family of highly conserved vertebrate-specific genes. Gene. 290(1-2):141-151.