PUBLICATION
Mechanisms underlying long- and short-range nodal signaling in Zebrafish
- Authors
- Jing, X.H., Zhou, S.M., Wang, W.Q., and Chen, Y.
- ID
- ZDB-PUB-060526-5
- Date
- 2006
- Source
- Mechanisms of Development 123(5): 388-394 (Journal)
- Registered Authors
- Chen, Yu
- Keywords
- Zebrafish, Nodal, Signaling range
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Body Patterning
- Embryo, Nonmammalian
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- Intracellular Signaling Peptides and Proteins
- Left-Right Determination Factors
- Molecular Sequence Data
- Nodal Signaling Ligands
- Protein Structure, Tertiary
- Signal Transduction*
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism*
- Zebrafish/embryology
- Zebrafish/metabolism*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism*
- PubMed
- 16701984 Full text @ Mech. Dev.
Citation
Jing, X.H., Zhou, S.M., Wang, W.Q., and Chen, Y. (2006) Mechanisms underlying long- and short-range nodal signaling in Zebrafish. Mechanisms of Development. 123(5):388-394.
Abstract
Precise regulation of the signaling range of secreted molecules is essential for proper pattern formation during development. The Nodal family of TGF-beta proteins has been shown to function as both short- and long-range signals. But the underlying mechanisms remain elusive. In this study, we investigated the regulation of the signaling range of zebrafish Nodal proteins Cyclops and Squint, which are short- and long-range signals, respectively. We show that (1) the stability of Cyclops and Squint correlates with the activity range but increasing the stability of the short-range Cyclops does not increase its signaling range; (2) structural differences in the N-terminus region of the mature peptides of Cyclops and Squint determine their differences in the signaling range and swapping the N-terminus region of the Squint mature ligand into that of Cyclops makes the latter function at a distance.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping