ZFIN ID: ZDB-PUB-060517-8
Cloning and developmental expression of the DEC1 ortholog gene in zebrafish
Yao, J., Wang, L., Chen, L., Zhang, S., Zhao, Q., Jia, W., and Xue, J.
Date: 2006
Source: Gene expression patterns : GEP   6(8): 919-927 (Journal)
Registered Authors: Wang, Lu, Yao, Jihua, Zhao, Qingshun
Keywords: Zebrafish, DEC1/Stra13/Sharp2, bHLH, Eye, Retinal pigment epithelium (RPE), Iris, Neural crest, Somite, Pineal gland (epiphysis), Heart, Common cardinal vein (CCV), Blood cells, Pronephric duct, Spinal cord, Notochord, Midbrain, Hindbrain, Liver, Gut, Pancreas
MeSH Terms:
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Basic Helix-Loop-Helix Transcription Factors/genetics*
  • Basic Helix-Loop-Helix Transcription Factors/metabolism*
  • Cloning, Molecular
  • Computational Biology/methods
  • DNA, Complementary/isolation & purification
  • Embryo, Nonmammalian
  • Gene Expression Regulation, Developmental*
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism*
  • Molecular Sequence Data
  • Tissue Distribution
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
PubMed: 16678499 Full text @ Gene Expr. Patterns
DEC1/STRA13/SHARP2 is a transcription factor of the bHLH family that has been suggested to play key roles in mammalian cell differentiation, the cell cycle and circadian regulation. However, the function of the DEC1 gene during embryogenesis is not well understood. In the present study, we cloned a zebrafish ortholog of the human DEC1 gene and analyzed its expression during development of zebrafish. The predicted protein encoded by zebrafish DEC1 consists of 403 amino acids, and shares 59%, 60% and 59% identity in overall amino acid sequence with human DEC1, mouse STRA13 and rat SHARP2, respectively. Zebrafish DEC1 contains a bHLH domain exhibiting 97% identity with that of the mammalian ortholog. During zebrafish embryogenesis, DEC1 is expressed in a strong ubiquitous manner before early segmentation. At 15-72hpf, DEC1 shows a specific and dynamic expression in the developing eyes, somites, pineal gland (epiphysis), heart, brain, spinal cord, notochord, pronephric duct, common cardinal vein and blood cells. In older zebrafish, DEC1 also is expressed in multiple tissues including the brain, eye, gut, liver and pancreas. Our data provide evidence that expression of DEC1 is evolutionally conserved in zebrafish.