PUBLICATION

Cloning and developmental expression of the DEC1 ortholog gene in zebrafish

Authors
Yao, J., Wang, L., Chen, L., Zhang, S., Zhao, Q., Jia, W., and Xue, J.
ID
ZDB-PUB-060517-8
Date
2006
Source
Gene expression patterns : GEP   6(8): 919-927 (Journal)
Registered Authors
Wang, Lu, Yao, Jihua, Zhao, Qingshun
Keywords
Zebrafish, DEC1/Stra13/Sharp2, bHLH, Eye, Retinal pigment epithelium (RPE), Iris, Neural crest, Somite, Pineal gland (epiphysis), Heart, Common cardinal vein (CCV), Blood cells, Pronephric duct, Spinal cord, Notochord, Midbrain, Hindbrain, Liver, Gut, Pancreas
MeSH Terms
  • Homeodomain Proteins/genetics*
  • Homeodomain Proteins/metabolism*
  • Gene Expression Regulation, Developmental*
  • Zebrafish/embryology*
  • Zebrafish/genetics*
  • Zebrafish/metabolism
  • Zebrafish Proteins/genetics*
  • Zebrafish Proteins/metabolism*
  • Amino Acid Sequence
  • Animals
  • Tissue Distribution
  • Cloning, Molecular
  • Computational Biology/methods
  • Basic Helix-Loop-Helix Transcription Factors/genetics*
  • Basic Helix-Loop-Helix Transcription Factors/metabolism*
  • Molecular Sequence Data
  • Base Sequence
  • Embryo, Nonmammalian
  • DNA, Complementary/isolation & purification
(all 19)
PubMed
16678499 Full text @ Gene Expr. Patterns
Abstract
DEC1/STRA13/SHARP2 is a transcription factor of the bHLH family that has been suggested to play key roles in mammalian cell differentiation, the cell cycle and circadian regulation. However, the function of the DEC1 gene during embryogenesis is not well understood. In the present study, we cloned a zebrafish ortholog of the human DEC1 gene and analyzed its expression during development of zebrafish. The predicted protein encoded by zebrafish DEC1 consists of 403 amino acids, and shares 59%, 60% and 59% identity in overall amino acid sequence with human DEC1, mouse STRA13 and rat SHARP2, respectively. Zebrafish DEC1 contains a bHLH domain exhibiting 97% identity with that of the mammalian ortholog. During zebrafish embryogenesis, DEC1 is expressed in a strong ubiquitous manner before early segmentation. At 15-72hpf, DEC1 shows a specific and dynamic expression in the developing eyes, somites, pineal gland (epiphysis), heart, brain, spinal cord, notochord, pronephric duct, common cardinal vein and blood cells. In older zebrafish, DEC1 also is expressed in multiple tissues including the brain, eye, gut, liver and pancreas. Our data provide evidence that expression of DEC1 is evolutionally conserved in zebrafish.
Genes / Markers
Figures
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Expression
Phenotype
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Mutations / Transgenics
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Human Disease / Model
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Sequence Targeting Reagents
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Fish
Fish
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Antibodies
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Orthology
Gene Orthology
bhlhe40
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Engineered Foreign Genes
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Mapping
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Citation
Yao, J., Wang, L., Chen, L., Zhang, S., Zhao, Q., Jia, W., and Xue, J. (2006) Cloning and developmental expression of the DEC1 ortholog gene in zebrafish. Gene expression patterns : GEP. 6(8):919-927.