header logo image header logo text
Downloads Login
General Information
ZFIN ID: ZDB-PUB-060517-30
Aromatase in zebrafish: A potential target for endocrine disrupting chemicals
Kallivretaki, E., Eggen, R., Neuhauss, S., Alberti, M., Kausch, U., and Segner, H.
Date: 2006
Source: Marine Environmental Research 62 Suppl 1: S187-90 (Journal)
Registered Authors: Neuhauss, Stephan
Keywords: Aromatase, Zebrafish, Endocrine disruption, Immunohistochemistry, Real time RT-PCR
MeSH Terms:
  • Animals
  • Antibodies/metabolism
  • Aromatase/biosynthesis
  • Aromatase/drug effects*
  • Aromatase/genetics
  • Aromatase/immunology
  • Blotting, Western/methods
  • Brain/enzymology
  • Brain Chemistry/drug effects
  • Gene Expression Regulation, Enzymologic/drug effects*
  • Gonads/enzymology
  • Immunohistochemistry
  • Male
  • RNA, Messenger/drug effects
  • RNA, Messenger/metabolism
  • Reverse Transcriptase Polymerase Chain Reaction/methods
  • Zebrafish/genetics
  • Zebrafish/metabolism*
  • Zebrafish Proteins/biosynthesis
  • Zebrafish Proteins/drug effects*
  • Zebrafish Proteins/genetics
  • Zebrafish Proteins/immunology
PubMed: 16698076 Full text @ Mar. Environ. Res.
In zebrafish, two isoforms of the aromatase gene exist, namely cyp19a1 and cyp19a2, expressed predominantly in the gonads and brain, respectively. In this study, we focus on characterizing the specificity of antibodies against the aromatase isoforms, and on (xeno)estrogen-induced changes of individual cyp19a2 mRNA concentrations in the brains of adult male zebrafish. Among three polyclonal antibodies studied, the one against CYP19A2 was found to be specific in Western blots and immunohistochemistry. Real-time RT-PCR analyses revealed strong interindividual variation of cyp19a2 levels in the brains of adult male zebrafish. After a three-week-exposure to (xeno)estrogens, mean values of cyp19a2 mRNA levels tended to increase, with significant induction at 200ng 17beta-estradiol/L, but interindividual variation of cyp19a2 expression was maintained.