PUBLICATION
Activation and roles of ALK4/ALK7-mediated maternal TGFbeta signals in zebrafish embryo
- Authors
- Sun, Z., Jin, P., Tian, T., Gu, Y., Chen, Y.G., and Meng, A.
- ID
- ZDB-PUB-060517-27
- Date
- 2006
- Source
- Biochemical and Biophysical Research Communications 345(2): 694-703 (Journal)
- Registered Authors
- Jin, Peng, Meng, Anming, Sun, Zhihui, Tian, Tian
- Keywords
- Zebrafish, Maternal, TGFβ, Smad2, Smad3, Activin, Nodal, Vg1, Mesoderm induction
- MeSH Terms
-
- Smad2 Protein/metabolism
- Gene Expression Regulation, Developmental/drug effects*
- Vertebrates/embryology
- Vertebrates/genetics
- Zebrafish Proteins/metabolism*
- Smad3 Protein/metabolism
- Cell Cycle/drug effects*
- Cell Cycle/physiology
- Mutation/genetics
- Activin Receptors/metabolism*
- Dioxoles/pharmacology*
- Zebrafish
- Signal Transduction/drug effects*
- Signal Transduction/physiology
- Transforming Growth Factor beta/antagonists & inhibitors
- Transforming Growth Factor beta/genetics
- Transforming Growth Factor beta/metabolism*
- Benzamides/pharmacology*
- Blastula/physiology
- Activin Receptors, Type I
- Animals
- Mesoderm/drug effects
- Mesoderm/metabolism
- PubMed
- 16696945 Full text @ Biochem. Biophys. Res. Commun.
Citation
Sun, Z., Jin, P., Tian, T., Gu, Y., Chen, Y.G., and Meng, A. (2006) Activation and roles of ALK4/ALK7-mediated maternal TGFbeta signals in zebrafish embryo. Biochemical and Biophysical Research Communications. 345(2):694-703.
Abstract
Activin, Nodal, and Vg1, members of the transforming growth factor beta (TGFbeta) superfamily, transduce signal through type I receptors ALK4 or ALK7 and play important roles in mesoderm induction and patterning during vertebrate embryogenesis. However, the timing and magnitude of the ALK4/ALK7-mediated maternal TGFbeta signals are not clear. SB-431542 is identified as an inhibitor of the ALK4/ALK5/ALK7-mediated TGFbeta signals and its specificity in vertebrate embryos has not been reported. We demonstrate that SB-431542 is able to specifically and reproducibly block the Smad2/3-mediated TGFbeta signals in zebrafish embryo. Embryos exposed to SB-431542 exhibit various defects phenocopying Nodal-deficient mutants. SB-431542 treatments starting at different cell cycles before the midblastula transition lead to different degrees of developmental defects in mesoderm induction and patterning, suggesting that maternal TGFbeta signals are activated right after fertilization and required for mesoderm formation and patterning.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping