PUBLICATION
Neural stem cells and neurogenesis in the adult zebrafish brain: Origin, proliferation dynamics, migration and cell fate
- Authors
- Grandel, H., Kaslin, J., Ganz, J., Wenzel, I., and Brand, M.
- ID
- ZDB-PUB-060517-12
- Date
- 2006
- Source
- Developmental Biology 295(1): 263-277 (Journal)
- Registered Authors
- Brand, Michael, Ganz, Julia, Grandel, Heiner, Kaslin, Jan, Wenzel, Isabell
- Keywords
- Adult neurogenesis, Proliferation zones, Neural stem cells, Danio rerio
- MeSH Terms
-
- Immunohistochemistry/methods
- Zebrafish*
- Cell Proliferation
- Bromodeoxyuridine/metabolism
- Age Factors
- Cell Movement
- Neurons/cytology*
- Neurons/physiology
- Cell Differentiation
- Animals
- Brain/cytology*
- Stem Cells/cytology*
- Stem Cells/physiology
- Proliferating Cell Nuclear Antigen/metabolism
- PubMed
- 16682018 Full text @ Dev. Biol.
Citation
Grandel, H., Kaslin, J., Ganz, J., Wenzel, I., and Brand, M. (2006) Neural stem cells and neurogenesis in the adult zebrafish brain: Origin, proliferation dynamics, migration and cell fate. Developmental Biology. 295(1):263-277.
Abstract
Lifelong neurogenesis in vertebrates relies on stem cells producing proliferation zones that contain neuronal precursors with distinct fates. Proliferation zones in the adult zebrafish brain are located in distinct regions along its entire anterior-posterior axis. We show a previously unappreciated degree of conservation of brain proliferation patterns among teleosts, suggestive of a teleost ground plan. Pulse chase labeling of proliferating populations reveals a centrifugal movement of cells away from their places of birth into the surrounding mantle zone. We observe tangential migration of cells born in the ventral telencephalon, but only a minor rostral migratory stream to the olfactory bulb. In contrast, the lateral telencephalic area, a domain considered homologous to the mammalian dentate gyrus, shows production of interneurons and migration as in mammals. After a 46-day chase, newborn highly mobile cells have moved into nuclear areas surrounding the proliferation zones. They often show HuC/D immunoreactivity but importantly also more specific neuronal identities as indicated by immunoreactivity for tyrosine hydroxylase, serotonin and parvalbumin. Application of a second proliferation marker allows us to recognize label-retaining, actively cycling cells that remain in the proliferation zones. The latter population meets two key criteria of neural stem cells: label retention and self renewal.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping