PUBLICATION
BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis
- Authors
- Gupta, S., Zhu, H., Zon, L.I., and Evans, T.
- ID
- ZDB-PUB-060508-13
- Date
- 2006
- Source
- Development (Cambridge, England) 133(11): 2177-2187 (Journal)
- Registered Authors
- Evans, Todd, Zon, Leonard I.
- Keywords
- Hematopoiesis, Endothelial, Pronephros, Transgenic, Zebrafish, lmo2
- MeSH Terms
-
- Animals
- Animals, Genetically Modified
- Biomarkers
- Bone Morphogenetic Protein Receptors/genetics
- Bone Morphogenetic Protein Receptors/metabolism
- Bone Morphogenetic Proteins/metabolism*
- Deoxyribonucleases, Type II Site-Specific/genetics
- Deoxyribonucleases, Type II Site-Specific/metabolism
- Gene Expression Regulation, Developmental
- Hematopoiesis*
- Kidney/blood supply
- Kidney/embryology
- Kidney/metabolism
- Mesoderm/metabolism*
- PAX2 Transcription Factor/metabolism
- Saccharomyces cerevisiae Proteins
- Signal Transduction*
- Somites/metabolism*
- Stem Cells/metabolism
- Time Factors
- Zebrafish/embryology
- Zebrafish/genetics
- Zebrafish/metabolism
- Zebrafish Proteins/metabolism
- PubMed
- 16672337 Full text @ Development
Citation
Gupta, S., Zhu, H., Zon, L.I., and Evans, T. (2006) BMP signaling restricts hemato-vascular development from lateral mesoderm during somitogenesis. Development (Cambridge, England). 133(11):2177-2187.
Abstract
The bone morphogenetic protein (BMP) signaling pathway is essential during gastrulation for the generation of ventral mesoderm, which makes it a challenge to define functions for this pathway at later stages of development. We have established an approach to disrupt BMP signaling specifically in lateral mesoderm during somitogenesis, by targeting a dominant-negative BMP receptor to Lmo2+ cells in developing zebrafish embryos. This results in expansion of hematopoietic and endothelial cells, while restricting the expression domain of the pronephric marker pax2.1. Expression of a constitutively active receptor and transplantation experiments were used to confirm that BMP signaling in lateral mesoderm restricts subsequent hemato-vascular development. The results show that the BMP signaling pathway continues to function after cells are committed to a lateral mesoderm fate, and influences subsequent lineage decisions by restricting hemato-vascular fate in favor of pronephric development.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping