PUBLICATION
Asymmetric localization of Numb:EGFP in dividing neuroepithelial cells during neurulation in Danio rerio
- Authors
- Reugels, A.M., Boggetti, B., Scheer, N., and Campos-Ortega, J.A.
- ID
- ZDB-PUB-060424-8
- Date
- 2006
- Source
- Developmental Dynamics : an official publication of the American Association of Anatomists 235(4): 934-948 (Journal)
- Registered Authors
- Boggetti, Barbara, Campos-Ortega, Jose, Reugels, Alexander, Scheer, Nico
- Keywords
- Danio rerio, zebrafish, neurulation, neuroepithelial polarity, spindle orientation, asymmetric localization, Numb, Numblike, PTB domain, PRR
- MeSH Terms
-
- Mutagenesis, Insertional
- Cloning, Molecular
- Embryo, Nonmammalian
- Neuroepithelial Cells/cytology
- Neuroepithelial Cells/physiology*
- Protein Structure, Tertiary
- Zebrafish Proteins/chemistry
- Zebrafish Proteins/genetics
- Zebrafish Proteins/metabolism
- Molecular Sequence Data
- Microscopy, Video
- Drosophila Proteins/chemistry
- Drosophila Proteins/genetics
- Drosophila Proteins/metabolism*
- Animals
- Nerve Tissue Proteins/chemistry
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/metabolism
- Zebrafish/embryology*
- Zebrafish/genetics
- Spindle Apparatus/physiology
- Reverse Transcriptase Polymerase Chain Reaction
- Cell Membrane/metabolism
- Gene Deletion
- Protein Isoforms/chemistry
- Protein Isoforms/genetics
- Membrane Proteins
- Introns
- Phylogeny
- Conserved Sequence
- Recombinant Fusion Proteins/chemistry
- Recombinant Fusion Proteins/metabolism
- Sequence Homology, Amino Acid
- Microscopy, Confocal
- Green Fluorescent Proteins/metabolism*
- Cell Polarity*
- Amino Acid Sequence
- Juvenile Hormones/chemistry
- Juvenile Hormones/genetics
- Juvenile Hormones/metabolism*
- PubMed
- 16493689 Full text @ Dev. Dyn.
Citation
Reugels, A.M., Boggetti, B., Scheer, N., and Campos-Ortega, J.A. (2006) Asymmetric localization of Numb:EGFP in dividing neuroepithelial cells during neurulation in Danio rerio. Developmental Dynamics : an official publication of the American Association of Anatomists. 235(4):934-948.
Abstract
In the neural plate and tube of the zebrafish embryo, cells divide with their mitotic spindles oriented parallel to the plane of the neuroepithelium, whilst in the neural keel and rod, the spindle is oriented perpendicular to it. This change is achieved by a 90 degrees rotation of the mitotic spindle. We cloned zebrafish homologues of the gene for the Drosophila cell fate determinant Numb, and analyzed the localization of EGFP fusion proteins in vivo in dividing neuroepithelial cells during neurulation. Whereas Numb isoform 3 and the related protein Numblike are localized in the cytoplasm, Numb isoform 1 is localized to the cell membrane. Time-lapse analyses showed that Numb 1 is distributed uniformly around the cell cortex in dividing cells during plate and keel stages, but becomes localized at the basolateral membrane of some dividing cells during the transition from neural rod to tube. Using in vitro mutagenesis and Numb:EGFP deletion constructs, we showed that the first 196 amino acids of Numb are sufficient for this localization. Furthermore, we found that an 11-amino acid insertion in the PTB domain is essential for localization to the cortex, whereas amino acids 2-12 mediate the basolateral localization in the neural tube stage.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping