ZFIN ID: ZDB-PUB-060424-6
Reconstruction of firing rate changes across neuronal populations by temporally deconvolved Ca2+ imaging
Yaksi, E., and Friedrich, R.W.
Date: 2006
Source: Nature Methods   3(5): 377-383 (Journal)
Registered Authors: Friedrich, Rainer, Yaksi, Emre
Keywords: none
MeSH Terms:
  • Action Potentials/physiology*
  • Animals
  • Biomarkers/analysis
  • Calcium/analysis
  • Calcium/metabolism*
  • Calcium Signaling/physiology*
  • Cations, Divalent
  • Electrophysiology/methods*
  • Fluorescent Dyes/analysis
  • Neurons/physiology*
  • Odorants
  • Olfactory Bulb/cytology
  • Olfactory Bulb/physiology
  • Spectrometry, Fluorescence
  • Staining and Labeling
  • Time Factors
  • Zebrafish
PubMed: 16628208 Full text @ Nat. Methods
Methods to record action potential (AP) firing in many individual neurons are essential to unravel the function of complex neuronal circuits in the brain. A promising approach is bolus loading of Ca(2+) indicators combined with multiphoton microscopy. Currently, however, this technique lacks cell-type specificity, has low temporal resolution and cannot resolve complex temporal firing patterns. Here we present simple solutions to these problems. We identified neuron types by colocalizing Ca(2+) signals of a red-fluorescing indicator with genetically encoded markers. We reconstructed firing rate changes from Ca(2+) signals by temporal deconvolution. This technique is efficient, dramatically enhances temporal resolution, facilitates data interpretation and permits analysis of odor-response patterns across thousands of neurons in the zebrafish olfactory bulb. Hence, temporally deconvolved Ca(2+) imaging (TDCa imaging) resolves limitations of current optical recording techniques and is likely to be widely applicable because of its simplicity, robustness and generic principle.