ZFIN ID: ZDB-PUB-060327-5
Conservation of RET Regulatory Function from Human to Zebrafish Without Sequence Similarity
Fisher, S., Grice, E.A., Vinton, R.M., Bessling, S.L., and McCallion, A.S.
Date: 2006
Source: Science (New York, N.Y.)   312(5771): 276-279 (Journal)
Registered Authors: Fisher, Shannon
Keywords: none
MeSH Terms:
  • Animals
  • Conserved Sequence*
  • Enhancer Elements, Genetic*
  • Gene Expression Regulation*
  • Humans
  • Models, Genetic
  • Neurons/metabolism
  • Proto-Oncogene Proteins c-ret/genetics*
  • Regulatory Sequences, Nucleic Acid*
  • Sequence Analysis, DNA
  • Takifugu/genetics
  • Transgenes
  • Zebrafish/embryology
  • Zebrafish/genetics*
PubMed: 16556802 Full text @ Science
Evolutionary sequence conservation is an accepted criterion to identify noncoding regulatory sequences. We have used a transposon-based transgenic assay in zebrafish to evaluate noncoding sequences at the zebrafish ret locus, conserved among teleosts, and at the human RET locus, conserved among mammals. Most teleost sequences directed ret-specific reporter gene expression, with many displaying overlapping regulatory control. The majority of human RET noncoding sequences also directed ret-specific expression in zebrafish. Thus, vast amounts of functional sequence information may exist that would not be detected by sequence similarity approaches.