PUBLICATION

Characterisation of duplicate zinc finger like 2 erythroid precursor genes in zebrafish

Authors
Hogan, B.M., Pase, L., Hall, N.E., and Lieschke, G.J.
ID
ZDB-PUB-060323-10
Date
2006
Source
Development genes and evolution   216(9): 523-529 (Journal)
Registered Authors
Hogan, Ben M., Lieschke, Graham J., Pase, Luke
Keywords
Hematopoiesis, Erythrocyte, Zinc finger, Znfl2, Znfl2b, Zebrafish
MeSH Terms
  • Animals
  • Erythropoiesis/genetics*
  • Gene Duplication
  • Gene Expression*
  • RNA, Messenger/analysis
  • RNA, Messenger/metabolism
  • Transcription Factors/analysis
  • Transcription Factors/genetics*
  • Zebrafish/genetics
  • Zebrafish/growth & development*
  • Zebrafish Proteins/analysis
  • Zebrafish Proteins/genetics*
  • Zinc Fingers*
PubMed
16532340 Full text @ Dev. Genes Evol.
Abstract
In separate expression pattern and micro-array screens the zinc finger containing factor, znfl2, has been previously implicated in hematopoiesis. Here we analysed znfl2 expression in detail and performed genetic epistatic analysis in a series of hematopoietic mutants and transient gain-of-function models. znfl2 expression in the hematopoietic intermediate mesoderm and derived erythrocytes required early genes cloche and spadetail, but not gata1. Expression was up-regulated in scl gain-of-function embryos, identifying znfl2 as an early erythroid factor that is regulated upstream or independently of gata1. Furthermore, we identified a duplicate znfl2 gene in the genome (znfl2b) which was expressed in early mesendoderm and weakly in the lateral plate mesoderm, overlapping in expression with znfl2. The production of loss-of-function models for znfl2, znfl2b and znfl2/znfl2b together suggested that these erythrocyte specific zinc finger genes are dispensible for erythropoiesis.
Genes / Markers
Figures
Expression
Phenotype
Mutation and Transgenics
Human Disease / Model Data
Sequence Targeting Reagents
Fish
Antibodies
Orthology
Engineered Foreign Genes
Mapping
Errata and Notes