PUBLICATION
Characterization of human gene encoding SLA/LP autoantigen and its conserved homologs in mouse, fish, fly, and worm
- Authors
- Wang, C.X., Teufel, A., Cheruti, U., Grotzinger, J., Galle, P.R., Lohse, A.W., and Herkel, J.
- ID
- ZDB-PUB-060315-8
- Date
- 2006
- Source
- World journal of gastroenterology 12(6): 902-907 (Journal)
- Registered Authors
- Keywords
- Autoimmune hepatitis, Autoantigen, Genomics, Bioinformatics
- MeSH Terms
-
- Zebrafish
- Animals
- Animals, Genetically Modified
- Drosophila melanogaster
- Adaptor Proteins, Signal Transducing/genetics*
- Proto-Oncogene Proteins pp60(c-src)/genetics*
- Introns
- Sequence Alignment
- Sequence Homology, Amino Acid
- Amino Acid Sequence
- Caenorhabditis elegans
- Exons
- Humans
- RNA, Messenger/genetics
- Conserved Sequence
- Evolution, Molecular
- Molecular Sequence Data
- Mice
- PubMed
- 16521218 Full text @ World J. Gastroenterol.
Citation
Wang, C.X., Teufel, A., Cheruti, U., Grotzinger, J., Galle, P.R., Lohse, A.W., and Herkel, J. (2006) Characterization of human gene encoding SLA/LP autoantigen and its conserved homologs in mouse, fish, fly, and worm. World journal of gastroenterology. 12(6):902-907.
Abstract
AIM:To approach the elusive function of the SLA/LP molecule, we have characterized genomic organization and conservation of the major antigenic and functional properties of the SLA/LP molecule in various species.METHODS:By means of computational biology, we have characterized the complete SLA/LP gene, mRNA and deduced protein sequences in man, mouse, zebrafish, fly, and worm.RESULTS:The human SLA/LP gene sequence of approximately 39 kb, which maps to chromosome 4p15.2, is organized in 11 exons, of which 10 or 11 are translated, depending on the splice variant. Homologous molecules were identified in several biological model organisms. The various homologous protein sequences showed a high degree of similarity or homology, notably at those residues that are of functional importance. The only domain of the human protein sequence that lacks significant homology with homologous sequences is the major antigenic epitope recognized by autoantibodies from autoimmune hepatitis (AIH) patients.CONCLUSION:The SLA/LP molecule and its functionally relevant residues have been highly conserved throughout the evolution, suggesting an indispensable function of the molecule. The finding that the only non-conserved domain is the dominant antigenic epitope of the human SLA/LP sequence, suggests that SLA/LP autoimmunity is autoantigen-driven rather than being driven by molecular mimicry.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping