PUBLICATION
Molecular characterization and expression analysis of stanniocalcin-1 in turbot (Scophthalmus maximus)
- Authors
- Shin, J., Oh, D., and Sohn, Y.C.
- ID
- ZDB-PUB-060315-11
- Date
- 2006
- Source
- General and comparative endocrinology 147(2): 214-221 (Journal)
- Registered Authors
- Keywords
- Teleost, Turbot, Stanniocalcin-1, STC-1, cDNA, mRNA expression, Tissue distribution, Recombinant protein
- MeSH Terms
-
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Western
- Cell Line
- Flatfishes/genetics*
- Flatfishes/metabolism
- Gene Expression Profiling
- Glycoproteins/genetics*
- Humans
- Molecular Sequence Data
- Phylogeny
- RNA, Messenger/metabolism
- Recombinant Proteins/metabolism
- Sequence Alignment
- Tissue Distribution/genetics
- PubMed
- 16524573 Full text @ Gen. Comp. Endocrinol.
Citation
Shin, J., Oh, D., and Sohn, Y.C. (2006) Molecular characterization and expression analysis of stanniocalcin-1 in turbot (Scophthalmus maximus). General and comparative endocrinology. 147(2):214-221.
Abstract
Stanniocalcin-1 (STC-1) is an anti-hypercalcemic hormone that is produced by the corpuscles of Stannius (CS) in the teleost. The hormone is a homodimeric glycoprotein involved in the calcium and phosphate regulation in both teleost fish and mammals. In the present study, we have cloned and characterized a full-length cDNA of STC-1 from the turbot (Scophthalmus maximus) CS and examined its expression pattern in various tissues. The turbot STC cDNA (1246 nucleotides) encodes a putative preprohormone of 248 amino acids (aa) with a signal peptide of 18 aa and a pro-sequence peptide of 14 aa followed by a mature protein of 216 aa. The deduced aa sequence of turbot STC-1 shows the highest sequence identity (86.3%) with that of European flounder STC-1 among the fish and mammalian STC-1. The aa sequence of turbot STC-1 showed moderate sequence identity with those of salmoniforms (chum salmon and rainbow trout), cypriniforms (zebrafish), ancient fish (gar and bowfin), and humans (43.7-66.9%), respectively, whereas it showed less identity with those of zebrafish and human STC-2 (23.8-25.4%). RT-PCR analysis revealed that the turbot STC-1 gene is expressed in the CS, pituitary, brain, kidney, liver, heart, muscle, and gonad. In a transient transfection of turbot STC-1 cDNA construct into human embryonic kidney 293T cells followed by Western blot analysis, a recombinant STC-1 secreted into a medium was detected as a single band of 28kDa under reducing conditions and a 50kDa protein under non-reducing conditions. Deglycosylation of the recombinant STC-1 led to a decrease in the molecular mass. Our data indicate that the turbot STC-1 gene is widely expressed and the secreted protein exists as a homodimeric glycoprotein.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping