ZFIN ID: ZDB-PUB-060306-6
HuC:Kaede, a useful tool to label neural morphologies in networks in vivo
Sato, T., Takahoko, M., and Okamoto, H.
Date: 2006
Source: Genesis (New York, N.Y. : 2000) 44(3): 136-142 (Journal)
Registered Authors: Okamoto, Hitoshi, Sato, Tomomi
Keywords: neuronal labeling, photo-conversion, transgenic, zebrafish
MeSH Terms:
  • Animals
  • Dendrites/physiology
  • ELAV Proteins/genetics*
  • ELAV-Like Protein 3
  • Embryo, Nonmammalian/metabolism
  • Gene Expression Regulation, Developmental/physiology*
  • Luminescent Proteins/genetics*
  • Microinjections
  • Nerve Net/cytology
  • Nerve Net/embryology*
  • Neurons/cytology*
  • Recombinant Fusion Proteins/metabolism
  • Staining and Labeling/methods
  • Transgenes
  • Trigeminal Ganglion/cytology
  • Trigeminal Ganglion/embryology*
  • Zebrafish/embryology
  • Zebrafish Proteins/genetics*
PubMed: 16496337 Full text @ Genesis
A simple and efficient procedure for labeling neurons is a prerequisite for investigating the development of neural networks in zebrafish. To label neurons we used Kaede, a fluorescent protein with a photoconversion property allowing conversion from green to red fluorescence following irradiation with UV or violet light. We established a zebrafish stable transgenic line, Tg(HuC:Kaede), expressing Kaede in neurons under the control of the HuC promoter. This transgenic line was used to label a small number of neurons in the trigeminal ganglion. Also, using embryos injected with the transgene, we labeled peripheral axon arbors of a Rohon-Beard neuron at 4 days postfertilization and observed the dendrite development of a tectal neuron for 3 days. These data indicate that Kaede is a useful tool to selectively label neural networks in zebrafish.