PUBLICATION
Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs
- Authors
- Giraldez, A.J., Mishima, Y., Rihel, J., Grocock, R.J., Van Dongen, S., Inoue, K., Enright, A.J., and Schier, A.F.
- ID
- ZDB-PUB-060302-6
- Date
- 2006
- Source
- Science (New York, N.Y.) 312(5770): 75-79 (Journal)
- Registered Authors
- Giraldez, Antonio, Inoue, Kunio, Mishima, Yuichiro, Rihel, Jason, Schier, Alexander
- Keywords
- none
- Datasets
- GEO:GSE4201
- MeSH Terms
-
- 3' Untranslated Regions
- Adenosine/metabolism*
- Animals
- Embryo, Nonmammalian/physiology*
- Embryonic Development*
- Female
- Gene Expression Profiling
- Gene Expression Regulation, Developmental*
- Genes, Reporter
- Green Fluorescent Proteins/genetics
- Green Fluorescent Proteins/metabolism
- MicroRNAs/physiology*
- Mutation
- Oligonucleotide Array Sequence Analysis
- Polymers/metabolism*
- Protein Biosynthesis
- RNA Stability
- RNA, Messenger/genetics
- RNA, Messenger/metabolism*
- Ribonuclease III/genetics
- Ribonuclease III/metabolism
- Transcription, Genetic
- Up-Regulation
- Zebrafish/embryology
- Zygote/physiology
- PubMed
- 16484454 Full text @ Science
Citation
Giraldez, A.J., Mishima, Y., Rihel, J., Grocock, R.J., Van Dongen, S., Inoue, K., Enright, A.J., and Schier, A.F. (2006) Zebrafish MiR-430 Promotes Deadenylation and Clearance of Maternal mRNAs. Science (New York, N.Y.). 312(5770):75-79.
Abstract
MicroRNAs comprise 1-3% of all vertebrate genes, but their in vivo functions and mechanisms of action remain largely unknown. Zebrafish miR-430 is expressed at the onset of zygotic transcription and regulates morphogenesis during early development. Using a microarray approach and in vivo target validation, we find that miR-430 directly regulates several hundred target mRNAs. Most targets are maternally expressed mRNAs that accumulate in the absence of miR-430. We also show that miR-430 accelerates the deadenylation of target mRNAs. These results suggest that miR-430 facilitates the deadenylation and clearance of maternal mRNAs during early embryogenesis.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping