PUBLICATION
Rab11-FIP4 is predominantly expressed in neural tissues and involved in proliferation as well as in differentiation during zebrafish retinal development
- Authors
- Muto, A., Arai, K.I., and Watanabe, S.
- ID
- ZDB-PUB-060210-4
- Date
- 2006
- Source
- Developmental Biology 292(1): 90-102 (Journal)
- Registered Authors
- Keywords
- Rab11-FIP4, Retina, Zebrafish, Proliferation, Differentiation
- MeSH Terms
-
- Animals
- Brain/cytology
- Brain/embryology
- Brain/metabolism
- Carrier Proteins/biosynthesis*
- Carrier Proteins/genetics
- Carrier Proteins/physiology
- Cell Count
- Cell Cycle/genetics
- Cell Cycle/physiology
- Cell Differentiation/genetics
- Cell Differentiation/physiology*
- Cell Proliferation*
- Cyclic AMP-Dependent Protein Kinases/biosynthesis
- Cyclic AMP-Dependent Protein Kinases/genetics
- Cyclin-Dependent Kinase Inhibitor p57/biosynthesis
- Cyclin-Dependent Kinase Inhibitor p57/genetics
- Hedgehog Proteins
- Nerve Tissue Proteins/biosynthesis*
- Nerve Tissue Proteins/genetics
- Nerve Tissue Proteins/physiology
- Oligonucleotides, Antisense
- Retina/cytology
- Retina/embryology*
- Retina/metabolism
- Signal Transduction/physiology
- Stem Cells/cytology
- Stem Cells/metabolism
- Trans-Activators/physiology
- Zebrafish/embryology*
- Zebrafish Proteins/biosynthesis*
- Zebrafish Proteins/genetics
- Zebrafish Proteins/physiology
- rab GTP-Binding Proteins/biosynthesis*
- rab GTP-Binding Proteins/genetics
- rab GTP-Binding Proteins/physiology
- PubMed
- 16457799 Full text @ Dev. Biol.
Citation
Muto, A., Arai, K.I., and Watanabe, S. (2006) Rab11-FIP4 is predominantly expressed in neural tissues and involved in proliferation as well as in differentiation during zebrafish retinal development. Developmental Biology. 292(1):90-102.
Abstract
Rab11 family interacting protein 4 (Rab11-FIP4) was initially identified in humans as an Rab11-binding protein, but its biological function has remained unknown. We cloned the zebrafish orthologue of Rab11-FIP4 (zRab11-FIP4) and analyzed its function in vivo by using antisense morpholino. zRab11-FIP4 was expressed as 2 alternative transcripts, i.e., the longer A-form predominantly expressed in neural tissues and the shorter B-form expressed ubiquitously; and in situ hybridization revealed that the A-form was the dominant form. In the developing retina, zRab11-FIP4 was expressed in progenitors throughout the retina at early stages; and then, along with the differentiation, the expression became gradually restricted to the ganglion cell layer and ciliary marginal zone. zRab11-FIP4A knockdown embryos exhibited eye phenotypes similar to those of the shh mutant, such as a small eye with impaired cell proliferation and the delay in cell-cycle exit and differentiation of retinal progenitors. The lack of induction of p57kip2 and enhanced expression of cyclin D1 were observed in the morphant retina. Importantly, the delay in cell-cycle exit was rescued by ectopic expression of either p57Kip2 or dominant-negative PKA, suggesting that Rab11-FIP4A plays pivotal roles in retinal development by regulating Shh signaling and a mechanism acting in parallel with Shh signaling in the control of cell-cycle exit.
Genes / Markers
Expression
Phenotype
Mutations / Transgenics
Human Disease / Model
Sequence Targeting Reagents
Fish
Orthology
Engineered Foreign Genes
Mapping